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Syk基因启动子甲基化和肝细胞癌术后复发转移的研究

The Study of Roles about Syk Promoter Methylation in Postoperative Recurrence and Metastasis of Hepacellular Carcinoma

【作者】 傅平

【导师】 曾庆黎;

【作者基本信息】 江西医学院 , 外科学, 2005, 硕士

【摘要】 目的探讨肝细胞癌组织Syk 基因启动子甲基化改变的特点,及其与肝细胞癌术后复发转移的关系。方法收集经病理诊断为肝细胞癌的外科手术切除标本64 例,其中根治性切除手术标本44 例,姑息性切除手术标本20 例。先提取肝细胞癌组织和癌周正常肝组织的基因组DNA,经亚硫酸修饰后,采用甲基化特异性PCR(methylation-specific polymerase chain reaction,MSP)方法检测Syk基因启动子甲基化改变。随访发现在64 例肝细胞癌患者中,有33 例术后发生复发转移。结合随访结果,探讨Syk 基因启动子甲基化和肝细胞癌术后复发转移的关系。结果⑴在64 例肝细胞癌组织标本中,有19 例检测到Syk 基因启动子甲基化,发生率为29.7%;在对应癌周正常肝组织中,只有2 例检测到Syk 基因启动子甲基化,发生率为3.1%。经统计学分析,肝细胞癌组织Syk 基因启动子甲基化发生率显著高于癌周正常肝组织Syk 基因启动子甲基化发生率。⑵在癌组织检测到Syk 基因启动子甲基化的19 例肝细胞癌患者中,有14 例术后发生癌复发转移,发生率为73.7%;在45 例癌组织未检测到Syk 基因启动子甲基化的肝细胞癌患者中,只有19 例术后发生癌复发转移,发生率为42.2%。经统计学分析,癌组织检测到Syk 基因启动子甲基化的肝细胞癌患者术后复发转移发生率显著高于癌组织未检测到Syk 基因启动子甲基化的肝细胞癌患者。结论⑴Syk 基因启动子甲基化可能是肝细胞癌发生发展过程中重要的机制之一。⑵肝细胞癌组织Syk 基因启动子甲基化可能是预测肝细胞癌患者术后复发转移潜在的生物标记物。

【Abstract】 Objective To detect methylation status of the promoter region ofSyk gene in hepatocellular carcinoma(HCC),the relationship betweenthe aberrant methylation of Syk gene and postoperative recurrenceand metastasis after resection of HCC was analyzed.Methods The samples of tumor and adjacent normal tissues wereselected from HCC patients(n=64).They all underwent resection,44cases underwent radical resection and 20 cases underwent palliativeresection. Total genomic DNA was extracted from tissue specimens,then DNA was treated with bilsulfite modification. Finally, usingmethylation-specific polymerase chain reaction technique (MSP),specimens from 64 HCC patients (tumor tissues, adjacent normaltissues) were detected methylation of Syk promoter region. Duringfollow-up, 33 cases were found to have postoperative recurrence andmetastasis. According to these data, we analyzed the relationshipbetween the aberrant methylation of Syk gene and postoperativerecurrence and metastasis.Results ⑴Among 64 HCC, methylation of Syk promoter region werefound in 19 patients(29.7%). In contrast, methylation of Sykpromoter region were found in only 2 of adjacent normal tissues(3.1%), the rate of methylation of Syk promoter in HCC was higherthan that of adjacent normal tissues. ⑵In 19 cases with Sykpromoter methylation, 14 were found to have postoperativerecurrence and metastasis(73.7%).In 45 cases without Syk promotermethylation, only 19 were found to have postoperative recurrenceand metastasis(42.2%). A significant difference was noted betweentwo groups .Conclusion ⑴There is significant difference of Syk promotermethylation between HCC and adjacent normal tissues. ⑵Promotermethylation of Syk gene may serve as a potential biomarker forpostoperative recurrence and metastasis of HCC patients.

  • 【网络出版投稿人】 江西医学院
  • 【网络出版年期】2005年 08期
  • 【分类号】R735.7
  • 【下载频次】87
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