【作者】 张达；

【导师】 田文儒；

【作者基本信息】 东北农业大学 ， 临床兽医学， 2005， 硕士

【摘要】 本研究根据热应激处理的强度和恢复时间不同,共分五个处理组:高温处理组:43℃(1h);连续热应激处理组:37℃(1h)—间隔0h—43℃(1h);三个不连续热应激处理组:37℃(1h)—间隔2h—43℃(1h);37℃(1h)—间隔4h—43℃(1h);37℃(1h)—间隔6h—43℃(1h)。按照上述处理组的要求对交配成功的小鼠实施热应激处理(热应激处理的时间为见阴栓当日8:00am,连续4d),通过小鼠的肛温、体重的失重、胚胎的发育情况和血液中谷胱甘肽浓度、活性氧活力和淋巴细胞百分数以及卵巢和子宫组织蛋白质表达情况的测定,结果表明: 1 在剧烈热应激前进行温和热应激预处理,对妊娠早期小鼠的保护作用与恢复时间密切相关,恢复时间在4h 左右其保护作用效果明显。2 不连续热应激预处理后昆明小鼠的血液中谷胱甘肽的浓度较之高温处理组和连续热应激处理组升高,而活性氧的活力降低。3 在剧烈热应激对妊娠早期母鼠及附植前胚胎损伤明显的情况下,热应激预处理从一定程度上提高了合子发育到囊胚的百分率;减少了胚胎早期发育过程中的畸形率;加速了早期胚胎发育过程;保证了热应激条件下胚胎早期发育过程中胚胎的数目(对比高温处理组)。4 不连续热应激预处理后,分子量为70kDa 的蛋白质在卵巢、子宫组织中的表达量增加。5 不连续热应激预处理后的昆明小鼠的肛温明显降低、体重的丢失量明显升高。6 热应激预处理对卵巢、子宫的重量无明显影响。7 热应激预处理对血液中淋巴细胞的数量无明显影响。更多还原

【Abstract】 In this experiment, mice were divided into six groups based on heat temperature and exposure time, In control group, mice were housed at 25℃and normal relative humidity(air RH).In a group treated with a acute heat stress , mice were exposed to a ambient temperature of 43℃for 1h with normal relative humidity. In four groups pre-treated with a same mild heat stress including a successive heat-stressed group and three discontiguous heat-stressed groups, mice were exposed to a ambient temperature of 37℃for 1 hour, and then recovered from 0 to 6 hours(0,2,4,6 hours) , immediately exposed to a ambient temperature of 43℃for 1 hour again. Mice with vaginal plug were heat-stressed on day 1 of pregnancy from 8:00 am to 9:00 am by my experimental protocol(coitus was verified by the presence of a vaginal plug),and the same treatment were used for successive 4 days. To study the effect of a mild heat pretreatment on mice during early pregnancy ,we carried out a measurement on rectal temperature, weight loss, embryos’shape , development stage and competence, GSH level, ROS activity and lymphocyte rates in blood, expressed an uncertain protein with a molecular weight of 70kDa.The results showed as follows: 1 Effect of pre-treatment with a maternal mild heat stress on mice exposed to actue heat environment link to recovery time .the optimal time range to achieve a protective reaction is about 4 hours post heating. 2 The GSH level in three discontiguous heat-stressed groups is lower than that in contol group,acute heat-stressed group and successive heat-stressed group,and ROS activity increased distinctively after pre-treatment with a mild heat stress,the difference was significant(p﹤0.05). 3 When the bad damage on early pregnant mouse and pre-implantation embryos is unavoidable,after the early pregnant mouse is treated with a mild heat stress, the proportion of embryos that developed to morulae or blastocysts and the number of mice embryos was significantly increased , abnormality rates of mice embryos was decreased distinctively,the stage ,the cleavage rate of mice embryos was accelerated obviously.(comparison with that in control group) 4 An uncertain protein with a molecular weight of 70kDa was largely expressed in three discontiguous heat-stressed groups compared to that in acute heat-stressed group and successive heat-stressed group. 5 After pre-treatment with a mild heat stress, the core temperature reduced from 2 hours to 6 hours post heating,the lowest temperature of mice rectum occurred 4 hours post heating,and the weight loss increased after 2 hours post heating. 6 Effect of pre-treatment with maternal heat stress on weight of ovary and uterus had no significant difference with that of control group. 7 Effect of pre-treatment with maternal heat stress on lymphocyte rates of blood had no significant difference with that of control group.更多还原