【作者】 陈家璧；

【导师】 陈仕平；

【作者基本信息】 福建医科大学 ， 外科学， 2005， 硕士

【摘要】 目的 研究一氧化氮合酶(NOS)三种亚型:神经型NOS(nNOS)、内皮型NOS(eNOS)和诱导型NOS(iNOS)在肾细胞癌(RCC)和正常肾组织中的表达情况,探讨它们在肾细胞癌发生、发展及预后的作用。 方法 采用免疫组织化学技术SABC法对60例肾癌组织标本和20例远离肾癌的正常肾组织标本(对照组)进行三种NOS染色,以细胞浆出现棕黄色染色为NOS表达阳性细胞。用图像分析进行定量分析比较,并与肾癌的病理分级作相关分析。 结果 1.iNOS在正常组及肾细胞癌组中均无表达。2.nNOS在正常组主要表达于肾小管上皮细胞胞浆,肾小球内也有零星分布的阳性产物;在肾细胞癌组主要表达于肿瘤细胞的胞浆。nNOS在正常组与肾细胞癌组表达的积分光密度值(×10~4)分别为2.22±0.22和2.14±0.19,P>0.05,两者之间差别无统计学意义。3.eNOS在正常组主要表达于肾小管上皮细胞胞浆,在肾小囊上皮细胞及肾血管内皮细胞有散在表达。eNOS在肾细胞癌组主要表达于肿瘤细胞胞浆。eNOS在正常组与肾细胞癌组表达的积分光密度值(×10~4)分别为2.66±0.22和2.32±0.20,P<0.01,两者之间差别有统计学意义,肾癌组与正常组相比eNOS的表达明显减少。eNOS在肾细胞癌中表达的积分光密度值与肾细胞癌的病理分级作Spearman秩相关分析,r=-0.823,P<0.01。表明eNOS在肾细胞癌中表达与肾细胞癌的病理分级呈负相关。 结论 1.iNOS在肾细胞癌和正常组织里均无表达,可能与肾细胞癌发生无关。2.nNOS在肾细胞癌的表达与对照组相比没有显著性差异,可能不参与肾细胞癌的发生过程。3.肾癌组织eNOS的表达明显低于对照组,两者有显著性差异;eNOS在肿瘤细胞中的表达与肿瘤级别呈负相关。提示eNOS可能参与肿瘤发生发展,可能成为肾细胞癌的一种预后指标。更多还原

【Abstract】 Objcetive To study the expression and significance of three kinds of nitric oxide sythase (NOS) in renal cell carcinoma (RCC) and evaluate their roles in the growth and prognosis of tumor.Methods Iramunohistochemically staining (SABC method) was performed to detect the expression of three kinds of NOS in 60 specimens of RCC tissues and 20 specimens of normal kidney tissues for contrast. The quantitative analysis was determined by image analysis technique. Then we studied the relationship between the expression of NOS value and the tumor clinical and pathological character.Results 1. No expression of iNOS was found in the tissue specimens of two groups. 2. The expression of nNOS was mainly seen in the renal tubule cytoplasma of normal kidney tissues, and in tumor cell cytoplasma of RCC. The integral optical density (IOD) of eNOS in normal group and RCC group was 2. 22 ± 0. 22 and 2. 14 ± 0. 19 respective , P> 0. 05. The difference was not statistically significant. 3. The expression of eNOS was mainly seen in the renal tubule cytoplasma of normal kidney tissues, and in tumor cell cytoplasma of RCC .The IOD of eNOS in normal group and RCC group was 2.66 ± 0.22 and 2. 32±0. 20 respective, P <0. 01. The eNOS value in tumor tissues was much lower than that in normal kidney tissues and was negatively related to the tumor stage and grade, r=-0.823, P < 0.01 .Conclusions 1. No expression of iNOS was found in the tissue specimens of two groups. It probably was not related to the tumor genesis. 2. The difference of nNOS value in two groups was not statistically significant which suggested that nNOS might not participate in pathogenic of RCC. 3.The eNOS value in tumor tissues was much lower than that in normal kidney tissues and was negatively related to the tumor stage and grade. Which suggest that eNOS maybe play a role in the tumor genesis and development.更多还原