【作者】 王中成；

【导师】 林新华；

【作者基本信息】 福建医科大学 ， 药理学， 2005， 硕士

【摘要】 目的:探讨库拉索芦荟中活性成分之一的芦荟多糖对创面愈合的影响和可能的作用机制,为开发用于促创面愈合的芦荟药物提供实验依据。方法:从库拉索芦荟的凝胶中分离、纯化出芦荟多糖,通过离体细胞培养实验研究芦荟多糖对成纤维细胞和表皮细胞生物活性的影响;制备以芦荟多糖为主药的外用软膏,并建立其质量控制方法;用小鼠建立浅Ⅱ度烫伤模型,研究自制软膏对创面愈合的影响,并探讨其作用机制。结果:离体细胞培养实验表明芦荟多糖浓度大于100μg/ml 时对成纤维细胞和表皮细胞的生长有显著的促进作用,呈现一定的量效关系,且无显著的毒副作用;成功制备了以芦荟多糖为主药的外用软膏,并建立了相应的质量控制方法;自制的外用软膏可以显著促进小鼠浅Ⅱ度烫伤创面的愈合,且未观察到对小鼠肝、肾功能有影响。结论:库拉索芦荟多糖对体外培养的成纤维细胞和表皮细胞的增殖以及小鼠浅Ⅱ度烫伤创面愈合具有显著的促进作用,且无毒副作用。因而,有望将芦荟多糖开发成新型、高效的促创面愈合药。更多还原

【Abstract】 OBJECTIVE:To elucidate the effect and possible mechanism of polysaccharideextracted from Aloe barbadensis Miller on wound healing and provide thepharmacological foundation of drug exploitation about wound healing of Aloe.METHODS:Polysaccharide was isolated and purified from Aloe barbadensis Millerand study its effect on fibroblast and epidermal cell cultivated in vitro then prepare theexternal use ointment and establish the quality control method. At last, study the effectand possible mechanism of ointment used on superficialⅡscald.RESULTS:A polysaccharide, which was mainly β(1→4)-D-acemannan, was isolatedfrom the fresh Aloe barbadensis Miller leaf. Cell cultivation in vitro results showed thatthe polysaccharide can promote the fibroblast and EC growth when Conc. higher than100μg/ml. The promotion effect depended on the AP Conc. in culture fluid, at the sametime, the results showed no distinguished difference. Then we successfully prepared theexternal use ointment involved AP and established the quality control method. Animalexperiment showed that the ointment could speed up the superficialⅡscald healing rate ofmice significantly without observating any side effects on liver and kidney.CONCLUSION:AP extracted from the fresh Aloe barbadensis Miller not only canpromote the fibroblast and EC growth but also can speed up the superficialⅡscaldhealing rate of mice. At the same time, the study proved the safety of AP. In a word, thestudy results show that AP may be exploitated to some new drugs about promoting woundhealing with optimistic perspective.更多还原