【作者】 冯苗；

【导师】 王自能；

【作者基本信息】 暨南大学 ， 妇产科学， 2005， 硕士

【摘要】 目的:本研究通过免疫组织化学技术和图像分析技术, 1.用胎儿造血干细胞和血管内皮前期细胞的共同抗原CD₃₄的抗体标志绒毛血管,观察比较葡萄胎及正常绒毛间质血管形态学特征。 2.检测葡萄胎及正常绒毛组织中血管生成因子如血管内皮生长因子（vascular endothelial growth factor,VEGF）及碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF）的组织定位及表达强度差别。 方法: 取孕6-9周人流绒毛（n=14）、部分性葡萄胎（n=27）、完全性葡萄胎（n=11）,行常规HE染色和免疫组织化学染色,并利用图像分析技术定量分析CD₃₄,VEGF与bFGF的表达强度。 结果: 1.绒毛间质微血管密度（microvessal density,MVD）高低依次为:正常早孕组)部分性葡萄胎组)完全性葡萄胎组。 2.在实验中几乎所有的完全性葡萄胎标本,仍然可见为数不少的CD₃₄标志的绒毛间质血管。在不同的葡萄胎标本,甚至同一个葡萄胎不同绒毛中血管的密度和结构变化很大,血管的管径不规则。总之,血管的密度和绒毛的水肿程度呈反比。随着孕周的增加,葡萄胎的MVD没有更多还原

【Abstract】 Purpose:1. To investigate the morphological characteristics of villous blood vessel of hydatidiform mole (HM) and normal early gestation.2. To explore the expression of vascular growth factor such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in villous stroma of HM.Methods:Vasculature was examined immunohistochemically in the villous stroma in 14 cases of normal early gestation placental villi ,27 cases of partial hydatidiform mole(PHM) and 11 cases of complete hydatidiform mole (CHM).The stain intensity and distribution of CD34、 VEGF and bFGF in these placental villi sample were evaluated via image analysis technique.Results:1. The microvessal density (MVD) in CHM group was statistically significantly lower than that of PHM group (p<0.01) .The MVD in PHM group was statistically significantly lower than that of normal early gestation group (p<0.01) .2. Antibody CD34 was able to detect the vasculature. Molar villi were stained by CD34. In the stroma of almost all molar villi examined, vessels were found, but their density and structure varied greatly in each molar villus even in the same CHM, and the vessels were irregular in diameter,In fully dilated molar villi the vessels often became narrow.Generally MVD was inversely proportional to the grade of the swelling of villi. No significant differences in vasculature were found in molar villi at different gestational ages.3. The content of CD34 in CHM group was evidently lower than that of PHM mole group (p<0.01) . The content of CD34 in PHM group was statistically significantly lower than that of normal early gestation group (p<0.01) .4. In villous stroma, the content of VEGF and bFGF in CHM group were statistically significantly lower than that of PHM group (p<0.01 and p<0.01, respectively) ; the content of VEGF and bFGF in PHM group were statistically significantly lower than that of normal early gestation group(p<0.01 and p<0.01, respectively) .Conclusions:1. Considerable vessels could be detected by CD34 in the villous stroma of CHM.2. The lower expression of CD34、 VEGF and bFGF in villous stroma might have relation with the defect of vascularization in HM.3. The disturbance of blood development of HM might cause fetaldeath.更多还原