【作者】 郑发武；

【导师】 吕来清；

【作者基本信息】 暨南大学 ， 人体解剖学， 2005， 硕士

【摘要】 目的 观察脑缺血后,针刺对脑梗塞大鼠缺血半影区TGF-β₁、GFAP表达的影响,为探讨针刺治疗脑缺血机制提供实验依据。 方法 用雄性SD大鼠采用大脑中动脉栓塞法建立大鼠脑缺血模型,分为缺血组和针刺治疗组。通过尼氏染色方法观察缺血和针刺后脑组织病理学改变,利用免疫组织化学染色方法观察针刺对额叶皮质、海马CA₁区TGF-β₁、GFAP表达的的调节。 结果 （1） 大脑中动脉栓塞后,尼氏染色可见针刺治疗组神经元损伤情况较相应缺血组明显减轻。 （2） 脑缺血后缺血侧额叶皮质及海马CA₁区TGF-β₁表达增加,且在7d达到高峰,不同时间点针刺治疗组与缺血组相比,TGF-β₁表达增加（P<0.05）; （3） 脑缺血后缺血侧额叶皮质及海马CA₁区、GFAP表达增加,且分别在3天和7d达到高峰,不同时间点针刺治疗组与缺血组相比,额叶GFAP在电针3d组比相应缺血组增多,针刺7d组比相应缺血组数目减少（P<0.05）,针刺14d组及缺血14d组基本无表达;海马CA₁区在针刺1d组、3d组和7d组比相应缺血组增多（P<0.05）,针刺14d组比相应缺血组减少（P<0.05）。 结论 脑缺血后TGF-β₁、GFAP表达均呈先上升后降低趋势,针刺上调TGF-β₁的表达,早期上调GFAP的表达,晚期下调GFAP的表达,可能是针刺治疗脑缺血损伤的机制之一。更多还原

【Abstract】 PurposeThis study was to observe the effects of acupuncture on TGF- β₁ and GFAP expression in cerebral ischemia rats, and to provide the experimental evidence for clinical treatment of acupuncture after ischemic cerebral damages.MethodsMCAO model was maded by intralumianal thread approach with Sprague-Daley（SD） male rats, and randomly divided into the acupuncture -treated group and untreated groups. Nissl staining was used to observe cerebral pathologic changes following ischemia and acupuncture.Immunohistochemical method was used to investigate the effects of acupuncture on TGF- β₁ and GFAP expression in ischemic penumbra at various time points after cerebral ischemia.Results（1） After middle cerebral artery occlusion（MCAO）, Nissl staining indicated the pathologic alterations occurred to a lesser degree in acupuncture -treated groups compared with untreated groups.（2） The number of TGF- β₁ positive cells in ischemic frontal cortex and hippocampus（CA₁）increased and reached the peak 7 days after ischemia, The number of TGF- β₁ positive cells of acupuncture treated groups was raised （P< 0.05）, compared with that of untreated groups.（3） The number of GFAP positive cells in ischemic rontal cortex and hippocampus（CA₁）increased and reached the peak 3 days and 7 days after ischemia, The number of GFAP positive cells in ischemic rontal cortex of 3 days acupuncture -treated groups was raised （P<0.05）, while in ischemic rontal cortex of 7 days acupuncture -treated groups was reduced （P<0.05）, compared with that of untreated groups. The number of GFAP positive cells in ischemic rontal cortex of 14 days acupuncture -treated groups and untreated was not expressed. The number of GFAP positive cells in hippocampus（CA₁） of l,3,7days acupuncture -treated groups was raised （ P < 0.05 ） ,while in hippocampus（CA₁） of 14days acupuncture -treated groups was reduced （P< 0.05） .ConclusionThe expression of TGF- β₁ and GFAP increased in the early period, while decreased in late period after cerebral ischemia. Acupuncture could upregulated TGF- β₁ expression and upregulated GFAP expression in early period while down regulated in late period. which might be one of the mechanisms of acupuncture against ischemic cerebral damage.更多还原