【作者】 陈黎；

【导师】 吴卫； 郑有良；

【作者基本信息】 四川农业大学 ， 生物化学与分子生物学， 2005， 硕士

【摘要】 鱼腥草原植物为三白草科蕺菜属蕺菜Houttuynia cordata Thunb.,其茎叶搓碎后有鱼腥味,故名鱼腥草。鱼腥草药蔬兼用,已被国家卫生部正式确定为“既是药品,又是食品”的极具开发潜力的植物资源之一。四川为鱼腥草的主要产区之一,在四川峨眉还发现蕺菜属一新种峨眉蕺菜Houttuynia emeiensis Z.Y.Zhu et S.L.Zhang。在当地俗称白鱼腥草,也作鱼腥草药蔬兼用。本研究对来源于四川不同地区的鱼腥草资源游离氨基酸、总氨基酸进行了反相高效液相色谱(RP-HPLC)分析,对其挥发油进行了薄层色谱(TLC)分析。主要研究结果如下: 1.对19份川产鱼腥草材料阴干地上部分游离氨基酸组成及含量进行了反相高效液相色谱分析。结果发现,分析的17种氨基酸中,6月和10月采收的鱼腥草中分别含有15种和14种氨基酸。氨基酸中脯氨酸含量最高,甘氨酸含量最低。10月采收的鱼腥草游离氨基酸总量高于6月采收者。此外,蕺菜与峨眉蕺菜的游离氨基酸成分间无显著差别。鱼腥草不同材料间游离氨基酸含量不尽相同,不同采收期游离氨基酸含量也有差异。游离氨基酸含量与染色体数目间相关不显著。 2.分别对16份川产鱼腥草材料幼嫩地上和地下部分的氨基酸成分进行了反相高效液相色谱分析。结果发现,鱼腥草不同材料的氨基酸含量不尽相同,同一材料幼嫩地上茎叶和地下茎的氨基酸含量也有差异,多以地上茎叶高于地下茎。在分析的17种氨基酸中,所有材料均含有16种氨基酸,都不含胱氨酸。地上部分的氨基酸中谷氨酸含量最高,组氨酸含量最低:地下部分的氨基酸中谷氨酸含量最高,酪氨酸含量最低。地上部分氨基酸总量以W01-16为最高,而地下部分氨基酸总量以W01-99为最高,分别为27.89mg/g和15.46mg/g。蕺菜与峨眉蕺菜的氨基酸成分间无显著差别。本试验还发现,同一植株部位中,具有相似化学结构和性质的氨基酸含量间通常具有较高的相关性:不同氨基酸在植株不同部位间的含量相更多还原

【Abstract】 HERBA HOUTTYNIAE, the whole plant of Houttuynia cordata Thunb., is a kind of traditional Chinese medicine and also a kind of delicious vegetable. Now it had been identified as one of the most potential resources which could be used as both medicine and food by State Health Department in China Plentiful Houttuynia resources were found in Sichuan Province. The new Species Houtluynia emeiensis Z.Y.Zhu et S.L.Zhang, locally called white Yuxingcao, was found at Emei Mountain. It was also used as a kind of Chinese medicine and also a vegetable at Emei and Leshan regions. In the study, to analyze the dissociation amino acid and total amino acid of Houttuynia accessions in Sichuan Province by High Press Liquor Chromatography (HPLC); to analyze the essential oil constituents of Houttuynia by Thin Layer Chromatography (TLC). The main results were as follows:1. The dissociation amino acid of the dry aerial parts of 19 Houttuynia accessions in Sichuan Province were analyzed by RP-HPLC. Among the analyzed 17 amino acids, 15 and 14 were found in the Houttuynia accessions harvested in June and October, respectively. The content of proline was the highest among all amino acid, while that of glycine was the lowest. Furthermore, The total amino acid content of the accessions harvested in October was somewhat higher than that havested in June. There was no significantly difference between the dissociative amino acid constituents of H.emeiensis Z.Y.Zhu et S.L. and Houttuynia cordata Thunb. . The dissociative amino acid contents of different Houttuynia accessions were different, so was the Houttuynia accessions harvested in two different periods. The correlation between the dissociative amino acid contents andthe chromosome number of Houttuynia accession was insignificant.2. The amino acid constituents and content of the young aerial parts and underground parts of 16 Houttuynia accessions in Sichuan Province were analyzed by RP-HPLC. The results showed that the amino acid contents of different Houttuynia accessions were different, so did the young aerial parts and underground parts of the same accessions. In most accessions, the total amino acid contents of the aerial stems and leaves were higher than that of underground stem. Among the 17 analyzed amino acids, 16 were found in the aerial stems and leaves and underground stems of all accessions. The amino acid cystine-ss-cystine was not found in any accessions. The content of glutamic acid was the highest among all amino acid in aerial parts, while that of histidine was the lowest. In underground parts, the content of glutamic acid was the highest among all amino acid, while that of tyrosine was the lowest. The total amino acid content of the accession W01-16 was the highest in aerial parts, while that of accession W01-99 was the highest in underground parts, with the value of 27.89 mg/g and 15.46 mg/g respectively. There was no significant difference between the amino acid constituents of H.emeiensis Z.Y.Zhu et S.L, and Houttuynia cordata Thunb.. The results also showed that there were higer correlationship between the contents of the amino acid with the similar chemistry structure and properties. The correlations between the contents of the different amino acid of the different parts of Houttuynia accessions were not the same. The results also showed that the correlations between the amino acids content of the young aerial parts and underground parts of Houttuynia accessions and the chromosome number was insignificant.3. The essential oil constituents of the fresh aerial, underground and dry aerial parts of 20 Houttuynia accessions in Sichuan Province were analyzed by TLC. According to the difference of the spot number and definition of the essential oil TLC pattern, the aerial parts of 20 fresh accessions were classified into 5 types, that of its underground parts into 3 types; and that of 19 dry aerial parts into 4 types. The essential oil constituents in the fresh aerial parts were different from that in underground parts.And the essential oil constituents that in the fresh plant were different from that in dry plant. The difference among the fresh accessions was more significant than that among the dry ones. And the differences among the aerial parts of the fresh accessions were more significant than that among the underground parts. There was a tendency that the spotnumbers increased with the chromosome number. The difference of essential oil constituents of H.emeiensis Z.Y.Zhu et S.L. and Houttuynia cordata Thunb. was insignificant.更多还原