【作者】 王微；

【导师】 祖元刚； 付玉杰；

【作者基本信息】 东北林业大学 ， 植物学， 2005， 硕士

【摘要】 本文采用超用超临界CO₂萃取技术提取甘草种子油,确定了最佳提取工艺,用GC-MS法对其挥发性成分和脂肪酸成分进行研究,并以其提油后固形物作为提取黄酮的原料。首次从乌拉尔甘草种子中鉴定了16种挥发性成分,以邻苯二甲酸二丁酯和烷烃类化合物为主;同时分离鉴定了5种脂肪酸成分,以具有多种活性且人体所必需的两种不饱和脂肪酸——亚油酸和α-亚麻酸为主,相对百分含量分别为24.3%和25.51%,因而,甘草种子具有重要的研究和应用价值。 采用水蒸汽蒸馏法提取甘草茎叶中挥发油,用GC-MS法分析其中的成分,首次分离鉴定了41种挥发性成分,以具有多种药理和生物活性的萜烯类化合物和多环芳烃为主,为其进一步的药理药效的研究奠定基础。 以乙醇为夹带剂,超临界CO₂法提取甘草茎、叶和提油后固形物中的黄酮类化合物,结果表明,甘草总黄酮含量叶>种子提油后固形物>茎,叶中黄酮含量达2.78%,为进一步开发利用甘草资源提供了依据。更多还原

【Abstract】 The seed oil of Glycyrrhiza uralensis Fisch was extracted by SFE-C0₂ and determined its best art. The chemical components were separated and identified by GC-MS and the residue after extraction of oil was used as material for the extraction of flavonoids. We identified 16 volatile constituents from this species for the first time, which were mainly Dibutyl phthalate and alkane; and identified 5 fatty acid after methylation of which 9, 12-0ctadecad.i enoic acid（Z, Z） and 9,12,15-Octadecatrienoic acid accounted for 24.3% and 25.51% of the relative content respectively. That indicates that the seed of Glycyrrhiza uralensis Fisch is of important value for study.The volatile oil of stem and leaf of Glycyrrhiza uralensis Fisch was obtained by steam distillation. The chemical components were separated and identified by GOMS and identified 41 volatile constituents from this species for the first time, of which were mainly terpene and polycyclophane and this provides foundation for further medical study. Using SFE-CO₂ for the extraction of flavonoids of different aerial parts of Glycyrrhiza uralensis Fisch with ethanol as cosolventshowed, the results showed that, leaf>seed residue after oil extraction>stem, and the flavonoid content in leaf reached 2.78%. This provides scientific material for further development and utilization of Glycyrrhiza uralensis Fisch.更多还原