【作者】 杨欢；

【导师】 陈钧；

【作者基本信息】 江苏大学 ， 生药学， 2005， 硕士

【摘要】 目的:研究黄连阿胶汤的化学组成及其对模型大鼠尿儿茶酚胺排泄量的影响。方法:运用气相色谱-质谱、高效液相色谱-电喷雾质谱、高效液相色谱等分析技术,研究了黄连、黄芩、白芍合煎液的化学成分及不同配伍方法对上清液中有效成分含量的影响,此外还探讨了人工胃液、人工肠液对合煎时产生沉淀物的水解作用;采用给予SD大鼠腹腔注射促皮质素的方法,建立了尿儿茶酚胺高排泄量模型大鼠,并将模型大鼠分为阴性对照组、对照药物组、低剂量组、中剂量组、高剂量组、替代药物组,分别灌胃蒸馏水,天王补心丸,低、中、高剂量黄连阿胶汤,首乌代鸡子黄供试药物,剂量为2mL/100g大鼠体重/日,连续15日,研究各组药物对模型大鼠病理改变的调控作用。结果:从合煎液的石油醚萃取物中鉴定出19个挥发性化合物,从合煎液的苯萃取物和甲醇提取物中推测出15个化合物;与单味药水煎液相比,合煎上清液中的小檗碱、黄芩苷和芍药苷含量分别降低了72.96%、81.92%、9.82%;沉淀经过人工胃液和人工肠液的水解后,在水解液中检出小檗碱和黄芩苷,未检出芍药苷,但是与单煎相比,仍然损失了62.14%的小檗碱和70.91%的黄芩苷。连续腹腔注射促皮质素四天后,大鼠的尿液总量平均增加了43.15%,尿NE排泄量平均增加17.85%,尿E排泄量平均增加49.90%,尿DA排泄量平均增加5.14%,而且大鼠出现精神亢奋、活动时间延长、易怒、不易抓取等行为学改变;施药期间,尿液总量显示了不同的变化趋势,各组大鼠的尿CAs排泄量均有不同程度的下降,施药结束后,中剂量组大鼠的尿液总量平均下降了20.44%,尿E、DA排泄量分别平均下降了41.76%和9.13%,高于阴性组和其它各施药组。结论:黄连、黄芩、白芍合煎液中含有微量挥发性成分及黄连生物碱、黄芩黄酮(苷)、白芍单萜苷等化合物;与单煎相比,合煎时小檗碱和黄芩苷的损失较多,即使将沉淀水解,也不能使小檗碱和黄芩苷全部还原,因此建议采用单煎后再混合的方式。模型大鼠有一定的自愈能力,天王补心丸抑制大鼠的自愈,中剂量黄连阿胶汤的作用最佳,替代药物与原方相比,疗效大为降低。黄连阿胶汤对模型大鼠显示良好的治疗效果的同时,也反证了给大鼠腹腔注射促皮质素造成的病理变化可能与阴虚火旺证有部分相似之处。更多还原

【Abstract】 Objections: To examine main chemical constituents and influence to model rats’ urinary catecholamines excretion of HuangLian-EJiao decoction. Methods: Having utilizing modern analytical technology including GC-MS, HPLC and HPLC-ESI-MS, to study chemical constituents of HuangLian, HuangQin, BaiShao’s whole .decoction and the influence of different cooperation methods to effective compounds in the supernatant. In addition, having researched the hydrolysis of the whole decoction’s deposition by artificial gastric juice and artificial intestinal juice. At the same time, having built up the model of high urinary catecholamines excretion by i.p. ACTH to SD rats, then the model was seperated to six groups, negative control group, contrastive medicine group, low dosage group, middle dosage group, high dosage group, alternative medicine group, and i.g. distilled water, Tianwangbuxin Wan, low, middle, high dosage’s Huanglian-Ejiao decoction and alternative medicine, 2mL/100g weight/day for 15 days, respectively. Results: Identified 19 volatile compounds and the other 15 compounds from petroleum ether, benzene and methanol extraction of the whole decoction in all. It shows that berberine, baicalin and paeoniflorin in the whole decoction’s supernatant decreased 72.96%, 81.92% and 9.82%, respectively, compared with the single decoction of HuangLian, HuangQin and BaiShao. Furthermore, after hydrolysis, both berberine and baicalin was detected but paeoniflorin. Nevertheless, compared with the single decoction, 62.14 percents of berberine and 70.91 percents of baicalin were losing yet. After i.p. ACTH for 4 days, average volumes of rat urine has increased 43.15%, besides urinary norepinephrin, epinephrin, dopamin excretion have increased 17.85%, 49.90%, and 5.14%, respectively, and that SD rats appeared some behavior’s changes including excitement, activity-increasing, irascibility, difficulty to snatch. During the oral administration, volumes of urine have showed different trend, and, urinary catecholamine excretion of each group have decreased on different degree. After administration for 15 days, average volumes of urine of middle dosage group decreased 20.44%, urinaryepinephrin, dopamin excretion decreased 41.76%, 9.13%, respectively, markedly highly to negative control group and other groups. Conclusions: There are a small quantity of volatile compounds and HuangLian alkaloids, HuangQin fiavones, as well as BaiShao monoterpenoids in the whole decoction. Compared with the single decoction, much of berberine and baicalin were losing during decocting wholly, and, even though the deposition have been hydrolyzed, only were small part of berberine and baicalin detected, consequently, writer suggest that it should be the better way that decocting the raw plant materials solely and then mixing the extraction. Rats model have showed self-cure ability, Tianwangbuxin Wan could inhibit that ability, and middle dosage HuangLian-EJiao decoction have the best effect, at the same time, compared with original prescription, the alternative medicine’s effect was highly weaken. HuangLian-EJiao decoction has showed the well curative effect to rats model, simultaneously, which, in turn, has demonstrated that pathological changes of SD rats model by i.p. ACTH could be similar to YinXuHuoWang Zheng of Chinese Medicine, partly.更多还原