【作者】 刘文娟；

【导师】 缪晓青；

【作者基本信息】 福建农林大学 ， 食品科学， 2005， 硕士

【摘要】 蜂王浆复方制剂(FSL)是以蜂王浆为主要原料,辅以药食两用的人参和灵芝。科学配伍制成的一种具有降血糖功能的保健新食品。本文主要就其配方的筛选,降血糖作用和急性毒性等进行了初步研究。 配方筛选的实验研究结果表明:人参乙醇同流提取条件较佳提取工艺为用5倍量于人参粉末的70%乙醇,提取4次,每次45min;灵芝水提煎煮提取条件较佳提取工艺为用15倍量于灵芝切片的蒸馏水,于100℃提取3次,每次lh,蜂王浆单方的剂量为2.4g/kg和1.5g/kg,人参为0.35g/kg和0.15g/kg,灵芝为0.09g/kg和0.24g/kg时降糖率较高.可作为下一步配方筛选的剂量组使用;当100g蜂王浆复合制剂中的含蜂王浆85g,灵芝9.6g,人参5.4g时,对高血糖动物的降血糖效果较好,为产品的较佳配方组成;蜂王浆复合制剂在冻干前加入等量的蒸馏水并进行相应的预处理,可得优质冻干粉——FSL;经有关机构鉴定.FSL含有丰富的营养成份。 对FSL降血糖作用的初步研究表明:FSL降血糖的有效剂量范围为300mg/kg—1000mg/kg。以FSL高(800mg/kg),中(500mg/kg),低(300mg/kg)剂量灌胃一次和连续灌胃10天,对正常小鼠血糖值无明显影响,初步显示FSL不会影响正常的糖代谢过程。FSL高、中、低剂量连续灌胃14天,对正常小鼠肝糖原含量无明显影响,但能明显改善正常小鼠的糖耐量。FSL高、中、低剂量组连续灌胃10天,能拮抗外源性肾上腺素、葡萄糖引起的血糖短暂升高。FSL高、中、低剂量连续灌胃14天,可显著降低四氧嘧啶所致糖尿病小鼠的高血糖并明显改善高血糖小鼠的糖耐量和增加其肝糖原含量。 毒理研究结果表明:FSL以最大浓度,最大体积给小鼠灌胃,均未出现动物死亡,测不出半数致死量(LDso)。测得小鼠的最大耐受量(MTD)为60.OOg/kg。 从上述研究结果,可以得出结论:FSL选用天然原料,经科学配伍制成,营养丰富,具有明显的降血糖作用,且毒性极低,可以作为一基本安全,有效的新型降血糖保健食品进行临床实验。更多还原

【Abstract】 Mainly containing royal jelly with Gen-seng and Ganoderma Lucidum as addition, glucose-lowering lyophilized royal jelly compound (FSL) is one kind of health-care food that has hypoglycemic effects. In the study the best formula of the compound, its hypoglycemic effects and acute toxin were investigated. Experiment on best formula showed that:Gen-seng can be best distilled when being put in 70% alcohol (5 times of Gen-seng) distilling for 4 times (45min per time) . Ganoderma Lucidum can be best distilled when being put in distilled water(15 times of Gen-seng) distilling for 3 times(1hour per time) at 100℃. Preparing experiments showed that royal jelly at dosage 1. 5g/kg, 2. 4g/kg, Gen-seng at dosage 0. 15g/kg, 0. 35g/kg, Ganoderma Lucidum at dosage 0. 09g/kg, 0. 24g/kg were most effective in lowering glucose respectively. So these dosage were fit for the next selection experiment. Before lyophilization, adding distilled water (the same volume of the mixture) in mixture of royal jelly, Gen-seng and Ganoderma Lucidum, then pretreating them, we can get high-quality preparation-FLS, which had abundant nutritious ingredient admitted by relevant authority.Primary study on FLS’ s hypoglycemic effects showed that: the effective glucose-lowering range of FLS was from 300mg/kg to 1000mg/kg. It had no significant effects on blood glucose of normal mice treated with high(800mg/kg), moderate (500mg/kg), low(300mg/kg) dosage of FLS once in lday or 10 suceesive days respectively. 14-suceesive days administration of FLS (high, moderate, low dosage) had no significant effects on liver glycogen of normal mice while it can significantly improve their glucose tolerance更多还原