【作者】 陈斌；

【导师】 彭荣章；

【作者基本信息】 南华大学 ， 泌尿外科， 2005， 硕士

【摘要】 目的:本实验通过建立大鼠同种异体肾移植模型,研究苦参碱的免疫抑制作用及其与环孢素A的协同作用,探讨苦参碱的免疫抑制机理,以求为苦参碱的深层次开发利用提供相关理论依据,并探讨苦参碱与环孢霉素A的协同作用关系及机理。 方法:建立大鼠同种异体肾移植急性排斥反应模型组与对照组,急性排斥反应模型组采用大鼠同种异基因肾移植模型(近交系SD→近交系Wistar)48只;对照组采用大鼠同基因肾移植模型(近交系Wistar→近交系Wistar)12只。对照组采用生理盐水1ml/kg.d腹腔注射,同种异体肾移植急性排斥反模型按随机原则分4组,每组各12只,分别为生理盐水组(生理盐水1ml/kg.d腹腔注射)、苦参碱组(苦参碱10mg/kg.d腹腔注射)、环孢霉素A组(环孢霉素A2.5mg/kg.d腹腔注射)、苦参碱+环孢霉素A组(苦参碱10mg/kg.d+环孢素A2.5mg/kg.d腹腔注射)。术后观察24小时尿量及体重变化情况,术后第7天从每组随机抽取6只麻醉后,取下腔静脉血测血Bun及Cr,并以ELISA法测定静脉血中IL-10及CD44水平,同时取移植肾组织行病理学检查。各组余下的6只大鼠继续以上述因素处理,直到死亡,以观察生存时间及24小时尿量。 结果:对照组大鼠存活时间均大于100天。生理盐水组平均生存时间为8.00±1.79天。CsA组的平均生存时间为10.50±1.22天。苦参碱组的平均生存时间为10.00±1.41天。苦参碱+CsA组大鼠最长存活时间延长至20天,平均生存时间为19.00±2.19天。苦参碱组与生理盐水组比较存在明显差异(p<0.05),Mat组与CsA组不存在明显差异(p>0.05)。苦参碱+CsA组存活时间与苦参碱组及CsA组存在明显差异(p<0.05)。各组与生理盐水组比较,24小时尿量明显增加,血Bun,Cr的水平各组间存在明显差异,其中生理盐水组最高,血Bun,cr的水平分更多还原

【Abstract】 Objective:To establish rat renal transplantation of acute rejection models,it was discussed that the mechanism of Mat resists acute rejection of renal allograft and synergistic effects with cyclosporine A (CsA). The immunosuppressive efficacy of Mat was discussed in order to improving relating theory-basis for further developing and the machanism of synergistic effects with low-dose CsA.Methods:Establishing rat renal allograft models with acute rejection (Donor:inbored SD rat Recipient: inbored Wistar rat).48 recipients were divided in to 4 groups at random.Group NS was treated with NS(1ml/kg.d ip);Group CsA was treated with Cyclosporine A(2.5mg/kg.d ip);Group Mat was treated with Matrine (10mg/kg.d ip);Group Mat + CsA treated with Mat (10mg/kg.d ip) combination with CsA (2.5mg/kg.d ip).Establising isograft models (Wistar-Wistar) ,12 recipients as a group was used as a control and were treated with NS (1ml/kg.d ip).The allograft survival time and 24hr-urinary volume were observed.Allografts of 6 recipients from each group were killed at random at day 7 after renal transplantation ,vein blood was received to monitor levels of Cr and Bun ,levels of IL-10 and CD44 in vein blood were monitored by enzyme-linked immunosorbent assay (ELISA).The transplanted kidneys were used to observe the patholoyical changes.The survival time and 24hr-urinary volume of other recipients were observed .All these indicator were used to analyse the efficacy更多还原