川白芷规范化种植质量评价的研究

【作者】 钟世红；

【导师】 马逾英；

【作者基本信息】 成都中医药大学 ， 生药学， 2005， 硕士

【摘要】 为配合川白芷生产质量管理规范化的研究,本文在课题组前期工作基础上进行了品质评价研究的完善和深入,为建立川白芷规范化的质量评价标准提供了科学依据。 本文首次考查了川白芷药材大小与质量的关系,主成分测定结果表明根条细小的药材（三等品）质量较好,建议在制定药材商品等级标准时结合内在质量评价;首次对新鲜川白芷断面进行了观察,发现皮部所占比例与断面直径大小成反比,从形态组织学的角度解释了三等品有效成分含量较高的原因;首次对川白芷的茎、叶进行了较详细的组织学研究,找出了茎、叶的主要鉴别特征。重点观察了分泌组织的存在形式和分布特点,发现分泌道散在于叶脉、叶柄及茎的皮层、韧皮部和髓部的薄壁组织中。 采用《中国药典》2000年版重金属、砷盐检查法对川白芷药材中的重金属、砷盐进行了检查。所测样品的重金属含量全部低于10ppm;砷盐含量全部低于2ppm。为川白芷药材重金属及有害元素测定方法的选择和限量标准的制定提供参考。 采用高效液相色谱法对不同白芷样品进行了欧前胡素、异欧前胡素和水合氧化前胡素三种香豆素类成分的含量测定,分析比较了连续四年的测定结果,结合生产实际,建议测定川白芷药材中欧前胡素(C₁₆H₁₆O₄)和异欧前胡素(C₁₆H₁₄O₄)两种香豆素,其总量不得少于0.12%。 建立了紫外分光光度法测定川白芷药材中总香豆素的方法。选择300 nm作为测定波长。以欧前胡素为对照,对不同川白芷样品进行了总香豆素含量测定。该方法准确度高,精密度好,操作简便、快速,可用于川白芷药材的质量控制。分析测定结果,建议总香豆素含量以欧前胡素计,不得低于0.6%。 本文首次采用HPLC法对川白芷药材进行丁香豆素类成分的指纹图谱研究。建立了川白芷香豆素类成分指纹图谱分析方法。将川白芷样品分为熏硫与未熏硫两类,分别标定共有峰。使用本文建立的指纹图谱可用于鉴别白芷药材是否熏硫。对川白芷规范化种植中研究中不同试验样品进行了考查,以总峰面积进行质量分析,结果与各项含量测定一致,完善了川白芷质量评价工作。建立的指纹图谱分析方法稳定、可靠、重现性好,能全面地反映出样品香豆素类成分的整体情况,可以作为川白芷质量评价、控制的方法。对川白芷浆汁的香豆素类成分指纹图谱进行了分析,结果表明浆汁指纹图谱中的各主要色谱峰与药材一致,说明香豆素类成分主要存在于分泌组织中。更多还原

【Abstract】 A complete and deep study on the quality evaluation of Chuan Bai Zhi has been done to establish its evaluation standard of quality,on the basis of pre-effort of the project group.The relationship between size and quality of Chuan Bai Zhi was studied for the first time and the result of content determination showed that the samples of small size(the third grade) was better. So the quality should be considered when establish a criterion of commercial grade of Chuan Bai Zhi. The observation of transverse section of the fresh radix showed that the proportion of cortex was in an inverse proportion to the diameter. Which explained why the samples of third grade have more content in a view of morphology. The major identification characteristics of the stem and the leaves were firstly been found through histology study. The form and distribution of secretary tissue was observed specially, it exists separately in parenchyma of the leaf veim petiole and stem ,as secretary canal.The heavy metals and arsenic salt were examined by relatived methods of pharmacopoeia(2000 edition).The content of heavy metals of all samples was under 10ppm,and that of arsenic salt was under 2ppm. Which could be references to the choice of determination methods and establishment of limitation standard.Imperatorin , isoimperatorin and oxypeucedanin such three main coumarins in Chuan BaiZhi were determined by RP-HPLC.Suggested the sum of imperatorin and isoimperatorin shouldn’t lower than 0.12% connected to the determination of four years and produce conditions.An UV method was established for the determination of total content of coumarin in Chuan Baizhi. The Coumarin was detected at 300 nm against imperatorin as an index. This method is rapid, reproducible and accurate .It was suitable for measuring the total content of coumarin in Chuan Baizhi. Suggested the content of Coumarin in Chuan Baizhi shouldn’t lower than 0.6% compare to imperatorin.The article established fingerprint of coumarin of Chuan Baizhi for the first time. All the Chuan Baizhi samples were divided into two sort: unsulfured and sulfured ones.And identified each characteristic peaks separately. This method could used to distinguish wether the sample was sulfured. Compared the samples of different experiments of Chuan Baizhi GAP by the whole area of coumarin peaks, which showed the same results as content determination. This stable , reliable and repeatable fingerprint method can be used for the quality control of Chuan Baizhi. Analysed the secretion of Chuan Baizhi and compared its fringerprint with the radix . The result showed that the main characteristic peaks in the two fringerprint were same, which identified that the coumarin exists in the secretory tissues.更多还原