【作者】 李小年；

【导师】 董小萍； 张梅；

【作者基本信息】 成都中医药大学 ， 药物化学， 2005， 硕士

【摘要】 目的:食用土当归Aralia cordata Thunb.作为中药九眼独活的主流品种被西部一些省的地方标准所收载,仅限于生药鉴定和资源调查等方面,但从近20多年国外（尤其是日本）发表的文献来看,已经研究得比较透彻,所含的许多化合物具有多方面的药理活性,所以该课题旨在为以后该药材质量标准的完善做部分基础性研究工作。方法:以95%乙醇对药材回流提取,系统溶剂萃取分成极性不同的几部位,结合大孔吸附树脂和硅胶色谱柱分离出主要的化合物,再通过UV、IR、NMR、MS等手段对其进行结构鉴定;然后以分离出的代表性化合物作为对照品,对药材进行定性定量研究。结果:从正丁醇部位分离出5个化合物Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ,经结构鉴定,Ⅰ是聚碳酸二甲基二对苯酚甲烷酯,Ⅱ是十六酸,Ⅲ是贝壳杉烯酸,Ⅳ初步推断为一无机盐,具体结构有待进一步分析,Ⅴ是海松酸,其中化合物Ⅰ和Ⅱ为首次从该植物中分得;以HPLC法测定,药材中十六酸和贝壳杉烯酸的含量分别为0.9718 mg·g^-1和13.0505 mg·g^-1。结论:食用土当归正丁醇部位化学成分的研究作为该药材物质基础研究工作的一部分,为以后中药九眼独活质量标准的完善提供了一定的依据。更多还原

【Abstract】 Aim: Udo Rhizome and Root （Aralia cordata Thunb.） as the major breed of the traditional Chinese medicine Jiuyanduhuo were recorded into the local Traditional Chinese Medicine Criteria which only ascertained the drug botanical origin and source investigation in some provinces of western China. Through referring to some journals published adroad, especially in Japan, 20 years lately, the author found that the plant had been studied intensively and that its many chemical components had pharmacological activities in various aspects. So the intent of experiment is to set up the groundwork of the evaluative criterion in quality and quantity of the traditional Chinese medicine Jiuyanduohuo. Methods: The roots .of Aralia cordata Thunb. were extracted with boiling 95% ethanol to give a EtOH extract. The EtOH extract was partitioned between water and various organic solvent successively to obtain several fractions. The n-butanol fraction was chromatographed on the Macroporous absorbent resin column （H₂O-EtOH） and repeatedly chromatographed on the silica gel （SiO₂） column using several kinds of solvent systems and further purified by recrystallization using various organic solvent to get main compounds. The structures of these compounds were identified by means of UV, IR, NMR, MS et al Then the methods to detect and measure the representative compounds vhich were isolated from this crude plant were establish. Results: Five更多还原