补肾活血中药复方对STZ性糖尿病大鼠视路病理损害的影响及机理探讨

【作者】 何宇；

【导师】 谢学军；

【作者基本信息】 成都中医药大学 ， 中医眼科学， 2005， 硕士

【摘要】 目的:观察补肾活血中药复方对实验性糖尿病大鼠视路病理损害的影响,并探讨其作用机理。 方法:采用链脲佐菌素(streptozotocin,STZ)制作大鼠实验性糖尿病模型。实验大鼠随机分为7组:正常对照组、阴性对照组(模型组)、阳性对照Ⅰ组、阳性对照Ⅱ组、中药低、中、高剂量组。以免疫组化染色法显示视网膜、外侧膝状体(exteral geniculate body,EGB)、视皮质(visual cortex,VC)中胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、髓鞘碱性蛋白(Myelin basic protein,MBP)和神经营养因子—3(neurotrophic-3,NT—3)的表达,应用Mias-2000图形分析系统对其进行测定和分析。 结果:(1)在病程6个月时,糖尿病大鼠视网膜、EGB和VC中GFAP、MBP、NT-3的阳性染色均较正常对照组有不同程度的降低(P<0.05或P<0.01);(2)与阴性对照组比较,中药各剂量组视网膜、EGB和VC中GFAP、MBP、NT-3的阳性染色均有增高,尤以中药高剂量组明显(P<0.05或P<0.01);(3)在同等使用降糖灵的基础上,中药高剂量组视路GFAP、MBP、NT-3的阳性染色较阳性对照Ⅰ组明显增高(P<0.05或P<0.01)。 结论:STZ性糖尿病大鼠在高血糖持续6个月后,表现出视路神经元和胶质细胞免疫组织化学的病理改变。补肾活血中药复方能够诱导糖尿病大鼠视路星形胶质细胞发生反应性胶质化,促进神经纤维髓鞘结构的恢复,并增加视路神经营养因子的表达,从而减少糖尿病视路神经元的病理损害,改善视路的神经传导功能,这可能是补肾活血中药复方减轻糖尿病视功能损害的重要机制之一。更多还原

【Abstract】 Object: To investigate the influence of traditional Chinese medicine (TCM) for bushenhuoxue (补肾活血) on the pathologic damages of visual pathway in experimental diabetic rats and discuss its effect mechanisms.Methods: The model of experimental diabetic rats was induced by streptozotocin (STZ) .The rats were divided into 7 groups randomly : normal control group, negative control group (model group) , positive control group I , positive control group II and three TCM groups, including low dose, middle dose and hige dose. Expression of glial fibrillary acidic protein (GFAP), Myelin basic protein(MBP), neurotrophic-3(NT-3)in retina, exteral geniculate body (EGB) and visual cortex (VC) was shown by immunohistochemical staining, and was quantitated by Mias-2000 image analysis system.Results: (1)At the 6 months of diabetic course, the positive staining of GFAP, MBP, NT-3 in retina, EGB and VC was reduced to a greater or lesser degree compared with the normol control group (P<0.05 or P<0.01) . (2)Compared with the negative control group, the positive staining of GFAP, MBP, NT-3 in retina , EGB and VC was upregulated in all of the TCM groups, especially in the hige dose group (P<0.05 or P<0.01). (3)By using the same dose of jiangtangling (降糖灵 ) ,the positive stainning of GFAP, MBP, NT-3 in the high dose TCM group was obviously increased compared with the positive control group I (P<0.05 or P<0.01) .Conclusion: after continuous hyperglycemia for 6 months, the experimental diabetic rats showed immunohistochemical changes of neurons and glial cells in visual pathway. The TCM for bushenhuoxue could induce astroglias to generate reactive gliosis in visual pathway, promote recovery of myelin structures, and upregulate the expression of neurotrophic factors. Accordingly, it reduce the pathological damages of neurons and improve the conduct function of visual pathway. It may be one of the important mechanisms of the TCM for bushenhuoxue to relieve the damages of optical function caused by diabetes.更多还原