太阴病脾阳虚证的唾液菌群特征研究

【作者】 姜冬云；

【导师】 翟慕东；

【作者基本信息】 成都中医药大学 ， 中医临床基础， 2005， 硕士

【摘要】 目的:探讨脾阳虚病人和正常人口腔唾液菌群细菌种类及数量的差异,进而揭示脾阳虚证唾液菌群种类、数量、分布及特征性变化规律。进而审视脾阳虚证客观化诊断指标,开拓经方微观辨证研究的新思路和新方向。方法:综合参照国内权威的脾阳虚诊断文献,制定本研究的临床实施标准,将确诊的脾阳虚证患者,纳入本实验。采用需氧、厌氧和真菌培养方法对脾阳虚病人口腔唾液样本进行定性和定量检测,并平行进行正常对照组唾液菌群比较研究。同时,对受试病人做血常规、血糖检查以及唾液淀粉酶活性检测,判断该证候的各个血常规指标、血糖水平和消化功能状况。结果:除真菌外,口腔各正常细菌的检出率(%)和CFU/ml较正常人均有不同程度的下降。其中,葡萄球菌、产黑色素的革兰阴性的无芽胞厌氧杆菌、奈瑟氏菌三种细菌在检出率(%)和CFU/ml上与正常人均有显著差别(p<0.05);唾液链球菌的检出率(%)和CFU/ml在脾阳虚组与正常组无明显差别(p>0.05),而采用同样方法完成的冠心病心阳虚组和家族性肾阳虚组,其链球菌在检出率(%)和CFU/ml等方面与正常组存在显著的差异。结论:葡萄球菌、产黑色素的革兰阴性的无芽胞厌氧杆菌、奈瑟氏菌检出率%和CFU明显下降(p<0.05)是太阴病脾阳虚证口腔菌群的基本特征之一;链球菌与正常组比较,未达到显著性差异(p>0.05)。此外,实验结果还显示太阴病脾阳虚证口腔菌群存在明显的菌群失调。更多还原

【Abstract】 Objects: Discuss the differences of bacterial categories and magnitude about saliva flora in mouth between Hyposplenoyang patients and healthy persons, and then open out the categories, magnitude, distributing and diversification laws of saliva flora of Hyposplenoyang Zheng . As well as contributing to establish new impersonally diagnostic standard of Hyposplenoyang Zheng. Furthermore, under the basis of it to carve out the new thoughts and research fields of microcosmic syndrome differentiation of classic prescriptions Methods: Referring to the authorized literature of diagnostic standard to Hyposplenoyang Zheng, early-constituted the clinic diagnose standard of this experiment, bring into the patients who has been diagnosed with it. Adopting the method of aerobic, anaerobic and fungicidal incubation to inspect the salva swatch of Hyposplenoyang patients with determing the nature and fixing quantify ration; and contrasing with that the normal person’ s. at the same time, checking the blood rutine, blood glucose and saliva amylase in order to estimate the respective items of blood rutine, the levl of blood glucose and the situation of digestive function. Results:The picking ratio (%) and CFU/ml of all normal bacteria in the mouth of Hyposplenayang patients absolutely have different extent declination except epiphyte. Thereinto, the picking ratio ( % ) and CFU/ml of staphylococcus, produce-melanin G~n non-sporangium anaerobic bacilus and neisseria have significantly differences between normal person (p<0.05): the picking ratio ( % ) and CFU/ml of saliva streptococcus has no significantly difference (p>0.05) between Hyposplenoyang group and normal group; with the same methods that had done to Hypocardioyang and Hyponephroyang group had significantly differences with normal group. Conclusion: The tincture of saliva flora of Taiyin Disease Hyposplenoyang Zheng as follows: the picking ratio (%) and CFU/ml of staphylococcus, produce-melanin G~n non-sporangium anaerobic bacilus and neisseria have significantly declined (p<0.05) , but the picking ratio (%) and CFU/ml of saliva streptococcus has no significantly difference (p>0. 05) contrasting with the normal group (p>0.05) .Furthermore, experimental results also indicate that the saliva flora of Taiyin Disease Hyposplenoyang Zheng has existed significant maladjustment.更多还原