【作者】 杨舸；

【导师】 王喜军；

【作者基本信息】 黑龙江中医药大学 ， 生药学， 2005， 硕士

【摘要】 柴胡(Radix bupleurum)为伞形科(Umbelliferae)植物柴胡[Bupleurum Chinense DC.]的干燥根及根茎。柴胡性平味苦、入肝、胆经。有发汗解表、疏肝解郁、升举阳气之功效。 本文对人工种植北柴胡的质量及其影响因素进行了研究。首先建立了人工种植北柴胡的质量评价方法,即采用高效液相色谱法结合比色法,以柴胡皂苷a、c、d为指标结合柴胡总皂苷含量综合评价的方法。并用该法对栽培措施、产地、采收期、加工方法等影响北柴胡质量因素进行研究。 对北柴胡生产过程中各影响因素的研究中,首先研究了栽培措施对北柴胡质量的影响。其中种苗分级实验结果表明,三级苗中柴胡主要有效成分含量最低,故建议间苗时除去弱苗;种植密度实验结果表明,种植密度为50株/m~2时北柴胡中有效成分含量较高,故认为北柴胡种植的密度为50株/m~2较佳;施肥实验结果表明,同时施加NP肥时有效成分含量最高,说明北柴胡生产过程中应该适时施加NP肥;对割蕾北柴胡有效成分积累研究表明,北柴胡中有效成分含量的最高时期在萌动期(4月初),且割蕾后北柴胡产量也有明显增加,故建议北柴胡绿果期(8月初)进行割蕾。同时我们对北柴胡生产过程中的几个关键环节进行了研究。对黑龙江省不同产区(七台河、北安、黑龙江中医药大学药用植物园)的规范化种植北柴胡研究表明,七台河产北柴胡质量最佳。因而,认为七台河为人工种植北柴胡最佳产地。不同采收期北柴胡有效成分积累研究表明,花期(7月中旬)柴胡皂苷a、c、d与柴胡总皂苷含量较高。但与割蕾北柴胡有效成分含量最高时期相比较,发现萌动期割蕾北柴胡有效成分含量最高。因而,认为北柴胡生产过程中应在花期割蕾,萌动期采收。对最适产地加工方法进行研究结果表明,阴干方法处理的北柴胡中有效成分与折干率均为最高,故北柴胡的最适产地加工方法为阴干。更多还原

【Abstract】 Chaihu the root of bupleurum Chinense DC. , belonging to the family of Umbelliferae, is the high qualified herbal drug grown in northeast part of China. It has the relieving exterior syndromes by diaphoriesis, soothing the depressed liver and improving the functional aspect of internal organs.In this paper, the method for evaluating the quality of cultivated Chaihu has been established by using RP-HPLC and spectrometry, saikosaponin-a, c, d and total saikosaponins were taken as the marker substance. By the method, the quality evaluation of various kinds of Chaihu, generated from different cultivating area, collecting time and processing method, has been carried out.As result, Chaihu cultivated in Qitaihe county has a much better quality than the others, Qitaihe was considered as the proper areas for cultivating the herbal drug. Among the samples collected at different seasons, that collected during the first 10 days of April has a highest content of the four marker substance, the collecting time was set on the early spring of the third growing year. For the processing method, drying at shade is the best selection. The determined results of the samples cultivated in different fertilized measures showed that there was no difference in different fertilized measures. The determined results of the samples in different grade showed that the first grade sample is the best in quality. The determined results of the samples cultivated in different density showed that the contents of saikosaponin-a, c, d and saikosaponins were hightest in the sample cultivated in density of 50 plant/m~2. The studies on the accumulated regulation of the contents of saikosaponin-acd and saikosaponins in topping更多还原