【作者】 陈海玲；

【导师】 李延；

【作者基本信息】 黑龙江中医药大学 ， 中医内科学， 2005， 硕士

【摘要】 目的:通过实验研究,探讨芪桂益脉灵防治快速性心律失常的作用机理,为中药治疗心律失常提供理论依据。 方法:选用Wistar种大鼠,雌雄不拘,200～250g。每组实验均选用健康大鼠50只,随机分空白对照组、生理盐水组、芪桂益脉灵Ⅰ组(25g生药/kg/次)、芪桂益脉灵Ⅱ组(50g生药/kg/次)、胺碘酮组(54mg/kg/次)5组,每组10只,每日清晨空服灌胃给药1次,连续7日。应用肾上腺素及西地兰诱发大鼠快速性心律失常模型,监测每组心律失常出现时间(OTR)、窦性心律恢复时间(RTSR)、西地兰中毒时间并采血测定SOD、MDA、Na~+-K~+-ATP酶的含量,观察芪桂益脉灵对肾上腺素、西地兰诱发的大鼠快速性心律失常的拮抗作用及对SOD、MDA、Na~+-K~+-ATP酶的影响。 结果:芪桂益脉灵与生理盐水组相对照,可明显延长肾上腺素诱发心律失常的发生时间(P<0.01),缩短窦性心律恢复时间(P<0.05),与胺碘酮作用结果相似。 芪桂益脉灵能延长大鼠对西地兰的中毒时间,并提高了大鼠对西地兰的耐受时间,与生理盐水相比有显著差异(P<0.01)。与胺碘酮比较,对西地兰的中毒时间无显著差异(P>0.05),但提高了大白鼠对西地兰的耐受时间(P<0.01)。 芪桂益脉灵组能显著提高超氧化物歧化酶(SOD)活性、降低丙二醛(MDA)含量,提高Na~+-K~+-ATP酶活性。与生理盐水组相比有显著差异(P<0.01),与胺碘酮组相比较有差异(P<0.05)。 结论:芪桂益脉灵对肾上腺素、西地兰诱发的心律失常均有较好的拮抗作用,说明芪桂益脉灵具有抗快速性心律失常的作用。其作用机制与抗氧自由基,减轻或消除心肌细胞因氧自由基所致的一系列损伤,保护心肌细胞,改善心肌代谢,提高心肌耐缺氧能力的作用有关。更多还原

【Abstract】 Objective: To research on the functions and mechanism of Qiguiyimailing(QGYML) in preventing tachy-arrhythmia; to supply the theoretical basis for treating of the tachy-arrhythmia by Chinese traditional medicine.Materrials andmethods: Select the white rats of Wistar kinds with the weight of 200-250g, without the difference of sex. Pick up 50 healthy rats for each group, divide them randomly into contrasting group, NaCl group, QGYML group I (25g herb/kg/time), QGYML group II (50g herb/kg/time), Amiodarone(54mg/kg/ 次) groups with 10 rats in each sub-group. Give the rats medicine every morning into stomach directly for 7 days. Apply Adrenaline and Lanatoside Digilanid C to the rats to stimulate the tachy-arrhythmia, then detect the OTR, RTSR, toxic time of Lanatoside Digilanid C of each group, determine the SOD, MDA, and the content of Na+-K+-ATPase enzyme by phlebotomizing, inspect the resisting function of QGYML to Adrenaline and Lanatoside Digilanid C , and the effects of it on SOD、 MDA、 Na+-K+-ATP enzyme.Results: By contrasting with the NaCl group, QGYML can obviously prolong the OTR (P<0.01) and shorten the RTSR (P<0.05), showing the similar results with Amiodarone.QGYM1 can prolong the toxic time of Lanatoside Digilanid C on rats, improve the living time of rats, representing the great difference (P<0.01) by comparing with NaCl. There is no big difference with Amiodarone on the toxic time, but it improves the living time of rats with Lanatoside Digilanid C (P<0.01).QGYML can improve the avidity of SOD and ATP, decrease the content of MDA, increase the avidity of Na+-K+-ATPase enzyme.更多还原