【作者】 陈盈盈；

【导师】 赛道建； 王子健；

【作者基本信息】 山东师范大学 ， 动物学， 2005， 硕士

【摘要】 随着科学技术的进步,工农业生产的发展,人类合成越来越多的化学品,水环境作为工农业废物的最终排放点,污染现象越来越严重。水体有毒有机物污染已成为世界各国科学界和政府关注的新热点。以往所采用的化学分析手段,虽然可以快速而灵敏地分析测定出污染物的种类和浓度及单一因子的直接影响,但因污染物种类繁多、数量巨大,不能反映污染物对生物和生态系统的影响。利用生命有机体对污染物的种种反应,来直接地表征环境质量的好坏及所受污染的程度的生物监测作为化学分析的补充手段是评价水质一个新的方案。采用基于细胞培养的生物方法监测和评估水质相比于藻类、鱼类等的生长繁殖试验具有费用低廉、节省时间、灵敏度高的优点,因此具有推广应用的前景。本论文通过细胞中性红实验、EROD 测试及重组基因酵母增殖实验三种生物毒性测试方法研究水环境中导致急性细胞毒性的物质、芳香烃(Ah)受体效应物质和内分泌干扰物质这几类有毒有机污染物,并选取了北京市北小河污水处理厂的进、出水及不同工艺出水和太湖梅梁湾表层沉积物这两种水环境样品进行测试,从而确定这三种生物测试方法是否适合批量测试水样。研究结果表明细胞中性红染色法(NR)能快速检测细胞毒性作用,数据处理方便、直观;EROD 生物测试所用的离体大白鼠肝癌细胞对类二恶英物质有着较高的灵敏性,2,3,7,8-四氯二苯并对二恶英(2,3,7,8-TCDD)与EROD 酶活力之间具有良好的剂量-效应关系,适合于对环境样品中的二恶英类化合物进行快速定量筛选;重组受体基因/报道基因的酵母检测技术则是一种筛选和定量分析环境样品中雌激素类污染物的快速、有效方法,本实验中重组基因酵母对雌激素类化合物也有较高的灵敏,17β-雌二醇标准化合物与β-半乳糖苷酶活性诱导的剂量-效应关系良好。在实际水环境样品测试中北京市北小河污水处理厂进、出水以及不同处理工艺出水的生物毒性测试表明城市污水厂出水中含有上述毒性特征的污染物,在再生水不同工艺深度处理中,微滤和反渗透工艺不能有效去除有毒有机污染物,超滤对具有急性毒性特征的物质和类雌激素物质去除效果最好,活性炭处理对Ah 受体效应物质的去除效果最好,这说明本实验所选取的生物毒性测试方法适于探讨再生水不同深度处理工艺技术的适用性,也适于水环境中有毒有机污染物的初步测评,如污水处理厂的不同处理工艺出水;用成组生物毒性测试方法对太湖梅梁湾表层沉积物有毒有机污染物毒性特征的背景值进行测试得知太湖梅梁湾的北部受到Ah 受体效应物质和内分泌干扰物质的严重污染,湖心则相对清洁,但南部且靠近湾中心受急性细胞毒性物质污染较重。这几类有毒有机污染物的分布规更多还原

【Abstract】 Aqueous environment is continuously loaded with foreign synthetic chemicals released byurban communities、agricultures and industries with the development of science technology.Recently, there has been increased concern regarding the role of aquatic environmental toxinorganic pollutants. The risk assessment of organic pollutants in environments is often basedon the concentrations detector by analytical chemistry and data on the toxicity of singlecompounds. However, all pollutants exert their effects as components of complex mixtures.The great number of compounds with their possible interactions makes it difficult to assessthe overall toxicity of effluents. Bioassays on aquatic concentrates provide a direct functionalresponse that relates to the overall toxic properties of the mixtures of compounds present in asample. Therefore, it is adaptable to evaluate water quality as a feasible scheme. Thecharacteristics of bioassays in vitro are low expenditure, time saving and high sensitivitycompared with the bioassay in vivo such as algae、fish reproducing experiments. In this paper,a battery of bioassays in vitro: neutral red, EROD and recombinant yeast bioassay wasstudied to assess the acute toxicants, Ah-acceptor chemicals and estrogenic substances inaquatic environments. Effluents from different advanced treatment units of the Bei Xiaohesewage treatment plant (STP) in Beijing and surface sediments from Meiliang Bay, TaihuLake were selected as the environmental samples.The results showed that neutral red assay is expeditious and the data processing isfacility. In EROD assay the H4IIE rat hepatoma cell used were sensitive to dioxin-likecompounds and there was a good dose-response relationship between 2,3,7,8-TCDD andEROD enzyme activity. This confirmed EROD assay in vitro is a rapid quantitative screeningfor the Ah-receptor-related toxicants in the environmental samples. Meanwhile therecombinant gene yeast assay technique is an efficient and rapid method for determining theactivity of estrogens in pollutants. In this study the recombinant gene yeast had highsensitivity to the estrogenic compounds and the yeast assay also had a good dose-responserelationship between 17β-estradiol and β-galactosidase enzyme activity as well. Additionally,更多还原