【作者】 徐卫团；

【导师】 崔乃强；

【作者基本信息】 天津医科大学 ， 中西医结合外科， 2005， 硕士

【摘要】 目的 建立一种研究胆汁分泌的慢性动物模型。方法 选用健康的Wistar大鼠,将其胆总管截断,近端插入直径0.8mm的“T”型硅胶管一长端,缝扎胆总管远端;硅胶管的另一长端插入空肠;建立皮下隧道,第三端引出体外,建成胆汁内、外引流的慢性动物模型。测定对照组、假手术组及模型组术后第1周以及胆道内、外引流后第1周的生化指标及体重的变化。结果对照组、假手术组、模型组术后第1周以及胆道内、外引流后第1周的生化指标及体重均无变化。结论 成功建立大鼠胆管内外引流模型。该模型为研究梗阻性黄疸提供了一个简单且可重复使用的研究平台,还可以观察药物对胆汁分泌的影响;对某些药物的胆汁浓度也提供了可靠的检测途径。更多还原

【Abstract】 Objective: To establish a chronic animal model of bile secretion. Method: In healthy Wistar rats, the common bile duct was transected ,with inserted into the long-terminal of a silica gel "T" tube of Φ =0. 8 mm in the proximal end, the other long-terminal of the "T" tube inserted into the jejunum. A hypo tunnel established, leading out the main portion of the "T" tube. Thus a rat obstructive jaundice model with bile drainage was established. The biochemical parameters and BM change of the first week after operation of control group, sham operation group and model group were determined. Results: No change was found in the biochemical indicator and BM change of the first week after operation of control group, sham operation group and model group. Conclusion: A rat bile duct internal and external drainage model was successfully established. The model provides a simple and更多还原