【作者】 王春丽；

【导师】 刘宗华； 汤继华；

【作者基本信息】 河南农业大学 ， 作物遗传育种， 2005， 硕士

【摘要】 以玉米杂交种农大108的F_2:3家系为基础材料,构建了具有179对SSR标记的玉米遗传图谱,标记间平均遗传距离为14.5cM,覆盖玉米基因组的2588.9cM。采用田间随机区组设计,考察了203个家系的穗长、穗粗、轴粗、穗行数、行粒数、百粒重、穗重和单株粒重等8个性状及不同时期的株高和叶绿素含量的动态变化情况,利用复合区间作图法对产量性状及有关农艺性状的数量性状基因位点（QTL）进行分析。 通过对玉米穗部性状、不同时期株高和叶绿素含量的动态分析得出以下结论: 1.对玉米穗部8个性状进行分析,除第7、10条染色体没有检测到QTLs以外,在其余8条染色体上,共检测到16个与产量性状有关的QTLs,其中穗重和单株粒重在新郑试点被检测到有共同的控制位点,为KW1（gw1）,位于第1条染色体上;穗粗在郑州和新郑两个试验点都被检测到有共同的位点为Ed2-1（Ed2-2）,分布在第2条染色体上。 2.对4个不同时期玉米株高进行分析,共检测到9个QTLs,分别位于玉米的6条染色体上,其中有4个QTLs在第4条染色体上,但位点所在区间不同,说明控制株高的QTL在不同时期的表达是不一样的。在非条件下共检测到7个QTLs,除了在8月1日没有检测到条件QTL,其余各时期都检测到了条件QTL;根据不同时期的净增长量数据,共检测到2个控制株高的QTLs。在两种情况下,不同时期没有被检测到的相同的QTLs,这些结果表明,控制株高的QTLs是以一定的时空方式表达。 3.对6个不同时期玉米叶绿素含量进行分析,共检测到10个QTLs。在非条件下,共检测到的7个QTLs大部分集中在第4、5条染色体上,其中在7月17日检测到的位点所在的区间与8月26日检测到的位点相同,为CC4,第4条染色体上,这可能是控制玉米叶绿素含量发育动态的同一个位点在不同时期表达的结果,表明在第4条染色体上存在控制叶绿素含量表达的位点;用不同时期条件数据进行QTL分析,结果也证明在第4条染色体上确实存在控制叶绿素含量表达的QTL位点。同时这充分说明不同时期玉米叶绿素含量受环境影响相对较小。更多还原

【Abstract】 Based on the 203 F_2:3 families of the hybrid ND108 in maize, a genetic linkage map contains 2588.9cM with 179 SSR markers was constructed, the interval distance was 14.5cM.The F_2:3 populations were evaluated in the farmer of Henan Agricultural Uuniversity and Xinzheng county with the randomized complete block design and two replications. The traits including ear length, ear diameter, axial width, row number, kernals per row, weight per hundred grains, ear weight, grain weight and the variety of plant height and chlorophyll content in different periods were investigated.QTLs were analyzed by composite interval mapping. The results were as following:1. At two locations （Zhengzhou and Xinzheng）, 16 QTLs were detected for 8 traits, locoted on all chromosomes except chromosome 7 and 9. The QTLs GW1 和 EW1 for ear weight and grain weight were detected on chromosome 1, There were the common QTLs and the QTLs Ed2-1 and Ed2-2 were identified for ear diameter on chromosome 2.They were also the common QTLs.2. The results of QTL mapping for maize plant height was showed that there were 9 different QTLs located on three chromosomes in the 4 different periods. Based on the analysis of the measured data, 7 QTLs were found in all period except for 1st August and accounted for 6.40% to 16.22%. No same QTL was detected in different periods; Based on the conditional value,2 QTLs with a little different effects were detected in different developing periods, which showed that the expression of QTL controlling plant height varied with the developing periods.The result implys that different QTLs controlling plant height expresses in different stage.3. QTL mapping and analysis of chlorophyll content have been made in the different periods, 10 QTLs were detected. In this investigation, using the measured data , chlorophyll content in leaf was measured. The results showed that 7 QTLs were detected for chorophyll content, which mainly distributed on chromosome 4 and chromosome 5,the interval of umcl288-umcl757 was detected by the value measured in both 17th July and 26th August on chromosome 4, their effects with a little bit different. But there were the common QTLs.This means that the expression of the same QTLcontrolling chorophyll content is different in different developing period.更多还原