【作者】 刘晟；

【导师】 邓松华； 汪昌荣； 王永杰；

【作者基本信息】 安徽医科大学 ， 病理学与病理生理学， 2005， 硕士

【摘要】 目的 观察严重烫伤大鼠早期血清心肌肌钙蛋白T(cTnT)、肿瘤坏死因子(TNFQ)的变化,心肌组织MDA、MPO的变化,并探讨葛根素对严重烫伤大鼠早期心肌损伤的保护作用。方法Wistar大鼠88只随机分为正常对照组(n=8)、复苏组(n=40)、治疗组(n=40),并在后两组中复制30%体表面积Ⅲ度烫伤动物模型。在烫伤后1、3、6、12和24小时,分别在各组中观察血清心肌酶谱(CK-MB)活性、心肌肌钙蛋白(cTnT)及肿瘤坏死因子(TNF-α)含量;以及心肌组织丙二醛(MDA)、髓过氧化物酶(MPO)含量。电镜下观察组织学形态变化。用SPSS统计软件分析上述指标在三个治疗组中的变化。结果 (1)在复苏组中,血清cTnT、TNF-α、心肌组织MDA、MPO含量在伤后1小时明显升高,均在伤后12小时达高峰,而CK-MB在伤后3h明显升高,其中cTnT升高达正常值的20倍。(2)在治疗组中,上述指标变化与复苏组趋势相同。但与复苏组相比均有显著降低。(3)心肌病理组织学超微结构改变提示心肌水肿,治疗组心肌病理改变明显轻于复苏组。结论 (1)严重烫伤后可引起心肌损害。(2)葛根素对烫伤早期心肌损害有保护作用。其机理可能是:①抑制炎症反应,减少机体炎症介质的产生与释放;②清除氧自由基,抗氧化;③抗缺血再灌注损伤等。更多还原

【Abstract】 Objective To observe the alteration of serum cTnT、 TNF-α 、 MDA contents and MPO activities in myocardium, and to investigate the protection of Puerarin (Pur) from the early myocardium damage of severe scalded rates. Methods A total of 88 Wistar rats were randomly divided into three groups: the control (n=8), the recovery (n=40) and the treatment (n=40), and in the later two groups a scald burn comprising 30 percent of the total body surface area full-thickness were established. The activity of serum CK-MB and the content of cTnT and TNF- α were detected in 1, 3, 6, 12 and 24 hours after the burn, as well as the content of MDA and the activity of MPO in myocardium . The morphologic changes were observed by electrical microscope. The indexes above were analyzed to acknowledge the variations in three groups with SPSS software. Results (1) In the recovery group cTnT、 TNF- α and MDA、 MPO went up 1 hours after burns, while CK-MB went up 3 hours after burns, and all attaining the top at 12 hours post burn. In cTnT, increasing about 20 times of the control group at 12 hours post burn. (2) In the treatment group, the indexs above had the same trends as the recovery group. But comparing with it, cTnT were much lower at 1, 3, 6, 12, 24 hours, TNF- α and CK-MB were much lower at 3, 6, 12, 24 hours, MPO and MDA were much lower at 1, 3, 6, 12 hours. (3) A series of ultrastractual changes in the myocardium were found at 3 and 6 hours postbum, the above changes attained the worst in 24 hours. Comparing with the recovery group, the pathological changes were obviously alleviated in the treatment group. Conclusions (1)The cardiadc myocytes damage occurred in early stage of seriously scald. (2)Pur protects the myocardium of seriously scalded rats in early stage. (3) The possible mechanism may be to inhibit inflammatory response and relieve the production and更多还原