【作者】 江燕；

【导师】 张洪福； 徐晓春； 曹立宇；

【作者基本信息】 安徽医科大学 ， 病理学与病理生理学， 2005， 硕士

【摘要】 背景与目的:苯并芘[Benzo(a)pyrene,B(a)P]是燃烧烟草烟雾的重要成分,Liang等用B(a)P和环氧二醇苯并芘[Benzo(a)pyrene diol epoxide,BPDE]处理食管正常和肿瘤细胞后克隆了170个BPDE结合DNA片段。本实验的研究目的是为了鉴定BPDE结合基因ATM(Ataxia-telangiectasia mutated)和EST#56(expressed sequence tag #56)作为食管癌早期诊断及其判断预后新生物标记物的可能性。方法:用原位杂交染色技术对63例食管鳞状细胞癌和52例食管正常黏膜进行ATM mRNA和EST#56mRNA的检测。结果:1.食管鳞状细胞癌中ATM mRNA表达率(63.5%,40/63)明显高于正常黏膜(26.9%,14/52)(p<0.05);ATM mRNA在食管鳞状细胞癌中的表达与肿瘤分化程度呈负相关(r=-0.312,p=0.013);ATM mRNA的表达吸烟组(80.6%,29/36)高于非吸烟组(40.7%,11/27)(p=0.001);而与患者的年龄、性别、临床分期、肿瘤大小、位置以及有无淋巴结转移无明显相关性(p>0.05) 2.食管鳞状细胞癌中EST#56 mRNA表达率(60.3%,38/63)明显高于正常黏膜(30.8%,16/52)(p<0.05)。EST#56 mRNA在食管鳞状细胞癌中的表达随着肿瘤分化程度的降低有逐渐升高趋势,但无统计学意义(r=-0.234,p=0.065)。EST#56 mRNA的表达吸烟组(72.2%,26/36)高于非吸烟组(44.4%,12/27)(p=0.049),而与患者的年龄、性别、临床分期、肿瘤大小、位置以及有无淋巴结转移无明显相关性(p>0.05)。3.食管鳞状细胞癌组织中ATM mRNA、EST#56 mRNA的表达呈明显正相关(r=0.531,p=0.001)。结论:1.吸烟能引起DNA受损导致基因突变而引起癌变,ATM参与了受损DNA的修复。2.EST#56基因可能是一抑癌基因,可能是烟草等环境致癌物的作用靶点,但还需进一步研究。我们还将进一步评价ATM和EST#56作为食管癌早期诊断和判断预后特别是放、化疗的生物标记物的可能性。更多还原

【Abstract】 Backgroud & Objective B[a]P is an important composition in tobaccosmoke.Liang et al have identified and cloned more than 170 of BPDE-binding DNA fragments after treatment of normal and cancerous esophageal cells with B [a] P and BPDE. Our research objective is to determine whether BPDE-binding gene ATM and EST#56 be used as novel biomarkers for early detection and prognosis of esophageal cancer. Methods In situ hybridization (ISH) was used to detect the expression of ATM mRNA and EST#56 mRNA in 63 cases of esophageal squamous cell carcinoma and 52 cases of the normal mucosae. Results 1. Expression of ATM mRNA in esophageal squamous cell carcinoma was higher than in the normal mucosae(63.5% vs 30.8%). Expression of ATM mRNA was inversely correlated with the degree of differentitation in tumors(r=-0.312, p=0.013).However,there is no correlation of ATM mRNA expression with age, gender, clinical stage , tumour size, tumour location and lymph node metastasis. Expression of ATM mRNA was higher in the smokers than in non-smokers (80.6% vs 40.7%). 2. Expression of EST#56 mRNA in esophageal squamous cell carcinoma was higher than in the normal mucosae(60.3% vs 26.9%). There was a trend toward higher EST#56 expression in different differentiated esophageal squamous cell carcinoma(r =-0.234, p=0.065).Expression of EST#56 was not found to be correlated with age, gender, clinical stage , tumour size, tumour location and lymph node metastasis. Expression of EST#56 mRNA was higher in the smokers than in non-smokers (77.8% vs 44.4%). 3. A positive correction was found between the expression of ATM mRNA and EST#56 mRNA (r=0.531, p=0.001).更多还原