【作者】 朱传凤；

【导师】 周健； 陈乔尔； 王元银；

【作者基本信息】 安徽医科大学 ， 口腔临床医学， 2005， 硕士

【摘要】 目的:探讨抑癌基因PTEN蛋白在口腔鳞状细胞癌(Oral Squamous Cell Carcinoma,OSCC)中的表达定位和表达水平,并与临床病理指标的关系及预后意义,及其分别与可能的下游基因:抑癌基因p27、癌基因cyclinD1的蛋白表达的之间的相关性。方法:用免疫组化S-P法检测54例口腔鳞状细胞癌、11例非典型增生组织、14例正常口腔粘膜组织中PTEN、p27及cyclinD1蛋白的表达,并与组织学类型、组织学分级、淋巴转移和临床分期等生物学行为进行相关分析。 结果:1.PTEN主要表达在细胞浆中。正常口腔粘膜中PTEN蛋白全部阳性表达,非典型增生组织PTEN蛋白阳性高表达率为90.9%,OSCC组织中PTEN蛋白的阳性高表达率为75.9%,其中高、中、低分化OSCC组织中PTEN蛋白的阳性高表达率分别为82.3%、76.1%和14.3%,在有、无淋巴结转移组织中PTEN蛋白的阳性高表达率分别为83.3%和41.7%。PTEN在OSCC组织中的蛋白表达与患者的性别、年龄、肿瘤大小、临床分期无明显相关性(P>0.05),但与组织类型、组织分化程度、淋巴结转移明显相关(P<0.05)。2.p27主要表达在细胞核,也有少量表达在细胞浆。在正常口腔粘膜组织、非典型增生组织、OSCC中p27高表达率分别为92.5%、90.9%和61.1%,其中高、中、低分化OSCC组织中p27蛋白的阳性高表达率分别为61.5%、47.6%和42.8%,在有、无淋巴结转移组织中p27蛋白的阳性高表达率分别为66.7%和41.7%。p27在OSCC组织中的蛋白表达与患者的性别、年龄、肿瘤大小、临床分期无明显相关性(P>0.05),但与组织类型、组织分化程度、淋巴结转移明显相关(P<0.05)。3.cyclinD1主要表达在细胞核,也有少量表达在细胞浆。在正常口腔粘膜组织、非典型增生组织、OSCC中cyclinD1高表达率分别为14.2%、18.2%和63%,其中高、中、低分化OSCC组织中cyclinD1蛋白的阳性高表达率分别为54.6%、71.4%和85.7%。cyclinD1在OSCC组织中的蛋白表达与患更多还原

【Abstract】 Objective :To study the localization and expression lever of PTEN,a tumor suppressor gene .And their relationship to p27 protein and cyclinD1 which are presumed downstream targets of PTEN,and clinical pathologic factors as well as the prognostic factors in 54 oral squamous cell carcinoma patients. Methods : The expression of PTEN ,p27,cyclinD1,was examined by immunohistochemistry S-P method in 14 cases of oral mucosa ,11cases of oral epithelial dysplasia and 54 of cases oral squamous cell carcinoma(OSCC). Results wese compared with clinical pathologic parameters ,and p27and cyclinD1 protein expression which were evaluated by immunohistochemical methods ,too.Results: 1. PTEN showed cytoplasm staining .Normal oral mucosa tissues was all positivefor PTEN protein . In the tissues from oral epithelial dysplasia ,90.9 % were positive for PTEN protein . In the tissues from OSCC ,75.9 % were positive for PTEN protein, there was no significant difference in the expression of PTEN in precancerous lesions (p>0.05).But ,there was significant difference in the expression of PTEN in cases of oral normal mucosa and oral squamous cell carcinoma .PTEN oncoprotein positive staining was found in 82.3% of well differentiared tumors,41.7%of moderately differentiated tumors and 14.3% of poorly differentiated tumors. PTEN oncoprotein positive staining was found in 83.3% of nolymph node metastasis, PTEN oncoprotein positive staining was found in 41.7% of lymph node metastasis. PTEN protein expression did not show correlation to sex and age, tumor size ,TNM stage (p>0.05), but there was a significant difference between well differentiated OSCC(82.3 % PTEN positive) and poorly differentiated OSCC(14.3. 3 % PTEN positive) (P < 0.05) .PTEN protein expression showed correlation to lymph node metastasis (P <0.05) . 2. p27 showed in nuclear.In the tissues from Normal oral mucosa tissues ,92.5 % were positive for p27 protein . In the tissues from oral epithelial dysplasia ,90.9 % were positive for p27 protein .In the tissuesfromOSCC ,61.1 % were positive for p27 protein. p27 oncoprotein positive staining was found in 61.5% of well differentiared tumors,47.6%of moderately differentiated tumors and 42.8% of poorly differentiated tumors. p27 oncoprotein positive staining was found in 66.7% of nolymph node metastasis, but p27 oncoprotein positive staining was found in 41.7% of lymph node metastasis. p27 protein expression did not show correlation to sex and age , tumor size ,TNM stage (p>0.05) but there was a significant difference between well differentiated OSCC(61.5%p27positive) and poorly differentiated OSCC(42.8% p27 positive) (P < 0.05) .p27 protein expression showed correlation to lymph node metastasis (jP<0.05) 3. Overexpression of cyclinDl immunostaining was mostly nuclear. In the tissues from Normal oral mucosa tissues 14.2 % were positive for cyclinDl protein . In the tissues from oral epithelial dysplasia ,18.2 % were positive for cyclinDl protein .In the tissues from OSCC ,63% were positive for cyclinDl protein. cyclinDl oncoprotein positive staining was found in 54.6% of well differentiated tumors,71.4%of moderately differentiated tumors and 85.7% of poorly differentiated tumors .cyclinDl oncoprotein positive staining was found in59.5% of nolymph node metastasis,but cyclinDloncoprotein positive staining was found in 83.3% of lymph node metastasis. cyclinDl protein expression did not show correlation to sex and age, tumor size ,TNM stage, lymph node metastasis (p>0.05) but there was a significant difference between well differentiated OSCC(54.6% cyclinDl positive) and poorly differentiated OSCC(85.7% cyclinDl positive) (P < 0.05 ) .4. Not only between overpression of PTEN and p27 but also between expression of PTEN and cyclinDl showed significantly positive correlation , and there was negative correlationship between p27 and cyclinDl.Conclusions : The higher incidence of negative expression of PTEN protein in OSCC indicates that PTEN gene inactivation may play an important更多还原