【作者】 石晓东；

【导师】 刘汉云；

【作者基本信息】 山西农业大学 ， 森林培育， 2005， 硕士

【摘要】 桃是世界性重要果树,其果色、香、味、质俱佳,很受消费者欢迎,在果品市场占重要地位,但桃受病毒危害非常严重。我国是桃主产国,桃病毒病同样给我国桃树生产带来巨大损失,严重制约了这一经济林木的发展。随着现代生物技术的发展,世界各国普遍采用组织培养方法进行苗木脱病毒的研究。 本文以山西农业大学林学院刘汉云副教授1991年从日本引进的川中岛白桃的带芽茎段为外植体,着重研究该品种离体培养的最佳培养基配方及培养程序。研究结果如下: 初代培养,外植体消毒采用0.5%NaClO处理20min效果较好。以MS+BA1.0mg/L+IBA0.1mg/L为最佳组合培养基配方。1月中旬为最佳取材季节。为防止污染,用500ppm浓度的硫酸链霉素或硫酸庆大霉素抑菌效果较好。 继代培养,最佳培养基为MS+BA1.5mg/L+IBA0.2mg/L,30d后其增殖率可达7.33。继代5次后,用改良MS代替MS可使材料诱导分化基本恢复正常。 生根培养,最佳培养基为1/2MS+IBA0.5～1.0mg/L+0.1%活性炭。20d后生根平均数为3.73～5.40条,生根率为29.8%～40.3%,生根最短时间为13.3 d。 炼苗以纯砂:珍珠岩=1:1为基质成活率最高,为52.0%。 本研究旨在建立川中岛白桃快速繁殖技术体系,以期为下一步桃脱毒苗木工厂化生产提供理论和技术依据。更多还原

【Abstract】 Prunus Persica was one kind of important fruit trees in the world. Peach used to be warmly received by the consumers and it played a great role in the fruit markets because of its beautiful color, sweet smiling and good quantities. But peach trees were badly harmed by virus. China was the biggest peach production country. The virus diseases also did a huge loss in peach production and limit the development of peach trees. Now in the world, with the development of modern biology techniques, tissue culture was mainly used for virus-free seedling research.In this paper, stem segments with bud of peach variety--white Chuznzhongdao peach were used forexplants. The variety was imported from Japan in 1991 by Liu Hanyun, vice professor of the Forestry Institute in Shanxi Agriculture University. The best media of white Chuznzhongdao peach in vitro culture and its culture procedure were mainly studied. The results were as follows:Primary culture medium: MS + BA 1.0 mg/L + IBA0.1 mg/L. The explants were effectively treated by NaClO (0.5%) for 20 min. Mid January was the best sampling season. In order to prevent pollution, 500ppm streptomycin or gentamycin sulfate could be used effectively.Subculture medium: MS+ BA 1.5 mg/L + IBA 0.2 mg/L. The propagation rate could reach about 7.33 for 30 days. After 5 times, the induction and differentiation came back normally, replacing MS by modified MS.Rooting medium: 1/2MS + IBA 0.5 - 1.0 mg/L + 0.1 %AC. 20 days later, the average of roots was up to 3.73-5.40, the rooting rate was 29.8% ~ 40.3%, the shortest rooting time is after 13.3 days.The highest survival rate of transplanted tube-seedling was 52.0%. The seedlings were transplanted in substratum made of sand and pearl ite (1:1).The purpose was to establish the tissue culture technique system of white Chuznzhongdao peach, providing for next productions of virus-free peach seedlings.更多还原