【作者】 李永辉；

【导师】 施启顺；

【作者基本信息】 湖南农业大学 ， 动物遗传育种与繁殖， 2005， 硕士

【摘要】 本研究依据人和小鼠同源基因保守序列设计引物,扩增出猪NCOA1基因的部分序列,利用PCR-RFLP方法检测了正虹种猪场454头外来猪种(大白、长白和杜洛克)NCOA1基因的RsaI酶切多态性,并分析了其对母猪产仔性状的影响;同时对该片段进行了序列测定、同源性比较和聚类分析。主要研究结果如下: 1.通过PCR-RFLP基因型检测发现,长白猪和大白猪均存在三种基因型:AA、AB、BB,且AA基因型的频率远高于AB型和BB型;而在杜洛克猪群中只检测到AA基因型,没有检测到AB型和BB型。并且在整个猪群中,AA基因型频率为0.7455,远远高于其他两种基因型的频率;A等位基因的频率(0.8613)远远高于B等位基因的频率(0.1387)。 2.利用SAS统计软件分析猪NCOA1基因与产仔数的关系表明:NCOA1基因的RsaI酶切多态性对母猪产仔性状具有显著的影响,在总产仔数(TNB)和产活仔数(NBA)上都出现AA>AB>BB的趋势,基因型之间差异极显著(P<0.01)。如大白猪AA基因型的平均总产仔数和产活仔数分别比BB基因型高2.66头和1.57头,在长白猪中AA型比BB型分别高2.28头和2.07头。说明NCOA1基因对产仔性状有显著影响,A等位基因为优势有利基因。 3.在对母猪胎次与产仔数的关系进行分析发现,母猪胎次对其产仔数有显著影响,头胎总产仔数、产活仔数均低于经产胎次。经产胎次的平均产仔数显著高于头胎(P<0.01);而NCOA1各基因型的产仔数在经产胎次中的差异也是显著的,AA型的母猪总产仔数和产活仔数显著高于BB型母猪(P<0.01)。但是对于头胎产仔数,长白猪基因型之间差异不显著(P>0.05),而大白猪基因型之间差异显著(P<0.05)。 4.本研究还对猪NCOA1基因扩增片段进行了序列测定,片段长度为440bp,并发现了一个T/C突变,该突变导致RsaI酶切多态性,但没有引起编码氨基酸的改变,为沉默突变。 5.对不同物种之间该序列的同源性比较,发现该序列在各物种之间具有较高的保守性;利用UPGMA法对猪、人、小鼠、大鼠、鸡、狗、黑猩猩共七个物种更多还原

【Abstract】 The NCOA1 primers were designed from well conserved-sequence region of homologous genes in human and mouse , a fragment was amplified from swine NCOA1 gene. PCR-RFLP procotol was employed to type 454 sows from 3 different foreign breeds in ZhengHong, the effects of them on sow litter size were analyzed. And the fragment was sequenced and analyzed. The main results are as follows:1. Through PCR-RFLP genotype detection, there are three genotypes in Landrace and Large White: AA, AB and BB, the frequency of AA genotype are far high than AB and BB genotypes; but there was only AA genotype found in Duroc, AB and BB genotypes were not detected. And in the whole sow population, the frequency of AA genotype was 0.7455,far high than the others. and the frequency of A allele (0.8613) are far more higher than B allele(0.1387).2. The correlation between NCOA1 gene and litter size were analyzed by using SAS software: the effects of NCOA1 polymorphism on swine litter size are significant, and there is a AA>AB>BB trend in TNB and NBA, the difference between genotypes are significant, the TNB and NBA of AA genotypes are the highest in Landrace and Large White , BB genotypes are the lowest. The TNB and NBA of AA genotype are 2.66 and 1.57 higher than BB genotype in Large White respectively, 2.28 and 2.07 higher in Landrace respectively. It shows that NCOA1 gene have great effects on litter size, A allele is an advantageous gene.3. The sow parity have great effect on litter size, the TNB and NBA of first parity are lower than still parity, and the average litter size in still parity are significantly higher than first parity (P<0.01); the differences between genotypes in still parity are significant, The TNB and NBA of AA genotype are significantly higher than BB genotype(P<0.01). in first parity, the differences between genotypes are not significant in Landrace (P>0.05 ) , but significant in Large White(P<0.05).更多还原