【作者】 林大专；

【导师】 王广树；

【作者基本信息】 吉林大学 ， 药物化学， 2005， 硕士

【摘要】 牛膝(Achyranthes bidentata BL)为苋科多年生草本植物,分布于除东北以外的全国广大地区。中药牛膝为其干燥的根,疗效好,产量大,是“四大怀药”之一。本文采用大孔树脂、1%NaOH 硅胶柱色谱等分离手段,从中药牛膝中得到6个单体化合物。通过测定理化常数及光谱分析等方法鉴定了化合物的结构,其中化合物C为首次从天然药物中得到的一个新甾体化合物,即: (2β,3β,5β,22R)–2, 3, 20, 22, 25-五羟基-胆甾-8,14-二烯-6-酮。化合物A、B、E、F为已报道的化合物,分别为: (2β,3β,5β,22R)–2, 3, 5, 14, 20, 22, 25-七羟基-胆甾-7-烯-6-酮(A);(2β,3β,5β,22R)–2, 3, 14, 20, 22, 25-六羟基-胆甾-7-烯-6-酮(B);3-O-β-D-吡喃葡萄糖醛酸基齐墩果酸-28-O-β-D-吡喃葡萄糖苷(E);3-O-β-D-吡喃葡萄糖基(1→2)-β-D-吡喃葡萄糖醛酸基齐墩果酸-28-O-β-D-吡喃葡萄糖苷(F)。化合物D还在鉴定中。更多还原

【Abstract】 Radix Achyranthis Bidentatae ,widely distributed in many places of China except for Northeast China, is known as a kind of Chinese traditional tonic herb and has many important biological activities , such as anti-fertility, anticancer, antibacterial, immune adjustment and anti-inflammatory activities. The constituents in Radix Achyranthis Bidentatae include triterpenoid saponins, polysaccharides, steroids, etc. The extraction and separation of steroid and triterpenoid saponin compounds from Radix Achyranthis bidentatae are carried out in this paper. Through using solvent extraction and column chromatography were 6 compounds isolated from Radix Achyranthis bidentatae. The structures of these compounds are elucidated by physico-chemical constants and spectroscopic analysis. They are: (2 β,3 β,5 β,22R)-2,3,5,14,20,22,25-heptahydroxy-cholest-7-en-6 -one(A); (2β,3β,5β,22R)-2,3,14,20, 22, 25-hexahydroxy-cholest-7-en-6-one(B); (2β,3β,5β,22R)-2,3,20,22,25–pentahydroxy-cholesta-8,14-dien-6-one(C); 3-O-β-D-glucuronopyranosyl oleanolic acid 28-O-β-D-glucopyranosyl ester(E); 3-O-β-D -glucopyranosyl(1→2)-β-D-glucuronopyranosyl oleanolic acid 28-O-β-D-glucopyranosyl ester(F). Compounds A、B、E、F have been reported, and compound C is new.更多还原