【作者】 杨晶；

【导师】 冯加纯；

【作者基本信息】 吉林大学 ， 神经病学， 2005， 硕士

【摘要】 缺血性脑损伤以其高发病率、高死亡率及高致残率而严重威胁人类的健康,越来越受到医学界的重视.本实验通过对高血压和非高血压大鼠慢性缺血模型进行免疫组织化学分析和行为学研究,试图探讨高血压、慢性缺血与痴呆的关系以及引起的白质病理改变。采用双侧颈总动脉永久结扎方法制备慢性前脑缺血大鼠动物模型,通过Morris水迷宫定向航行实验检测各组大鼠记忆能力,用免疫组化的方法分别检测GFAP、MBP、NF-H 三种成分的变化,进行图像分析。结果发现慢性缺血3 个月,单纯高血压组、单纯缺血组及高血压+缺血组MBP 和NFH 水平明显下降,而GFAP 增加,上述改变以高血压+缺血组尤为突出。比较MBP 和NF-H 的下降程度,MBP 变化比NF-H 的改变显著。行为学改变表明五组大鼠空间记忆能力皆有所下降,以高血压+缺血组下降明显。此研究表明慢性低灌注情况下,大鼠空间记忆能力下降。髓磷脂的损伤先于白质成分中轴突、胶质细胞的损伤,为慢性缺血时的首要病理变化。而上述改变在高血压条件持续存在时均加重。这一研究可以帮助我们理解慢性缺血和高血压时白质分子水平的代谢改变情况。更多还原

【Abstract】 Background and objects:The mechanism that chronic cerebral hypoperfusionconduced dementia is intricacy,this dementia is a ordinary little vasdementia .Clinic character is progressing dementia based on grainarteriosclerosis .Hypertension and diabetes may aggravate this progress.In thebrain of this animal model,rarefaction of white matter with dilatation of theventricles was frequently observed .To understand further the mechanism ofwhite matter changes under cerebral hypofusion and hypertension , chronologicalsequence of molecular changes in the cerebral white matter of the animal modelwas determined. Then investigating from pathology,immunohistochemistryanalyse and clinic intelligence examine aspects,trid to probe into the connectionthat hypotension、chronic hypofusion with white matter dementia and whathappened in white matter.Methods: Spontaneously hypertensive rats and non-hypotension rats were used inthis experiment,both carotid arteries was ligated.The levels of myelin basicprotein(MBP) as a marker of myelin,neurofilament H (NF-H)as a marker ofaxomal protein,and glial fibrillary acidic protein(GFAP) as a marker of astrogliawere analyzed after 3 months of cerebral hypoperfusion with Western blottingand enzyme-linked immunosorbent assay. Space memory of rats was registeredwith Morris water maze.Results:The result after 1 and 3 months of hypoperfusion showed that in theoperated group the levels of MBP and NF-H decreased and GFAP increased,inoperating plus SHR group is especially.The time course of MBP and NF-Hchanges revealed that the changes of MBP preceded that of NF-H .The spacememory of five groups were all declined,that was related with chronic cerebral更多还原