【作者】 赵晶；

【导师】 魏永巨；

【作者基本信息】 河北师范大学 ， 无机化学， 2005， 硕士

【摘要】 中草药是具有悠久历史的中华瑰宝,在中国医学史上发挥了巨大的作用,已成为中国文明的一部分。在科学技术日益先进的今天,如何用现代化的研究方法探索中草药的有效成分及其防病治病的作用机理,并建立标准化指纹图谱数据库,使中草药发挥更大的作用,已成为中草药研究的重要任务。荧光分析法在中药研究及其有效成分与生物大分子相互作用方面有着广泛的应用。特别是三维荧光光谱的发展,为中药指纹鉴别提供了广阔的应用前景。 本文将中草药作为一有机整体,利用荧光分析法对独活(RAP)、板蓝根(RI)、知母(RA)的整体荧光性质进行了研究。并在此基础上研究了这三种药物与血清白蛋白(SA)之间的相互作用。目的在于从整体上把握中药的光谱性质,并为进一步的药理研究奠定基础。 论文工作主要有三部分: 1.研究了独活的荧光光谱性质,考察了各影响因素。确定选用水提取液、煮沸10min配成储备液,进行测定。实验发现碱性条件下,独活存在两种荧光组分。研究了独活与BSA体系的荧光光谱和紫外光谱,发现二者之间存在能量转移和结合作用。考察了实验条件对体系的影响。研究了独活与HSA的相互作用。 2.研究了板蓝根水溶液的荧光光谱。实验考察了溶剂影响、光谱重现性以及酸度、浓度等方面内容。研究了板蓝根与BSA体系的荧光光谱和紫外光谱,发现二者之间存在能量转移和结合作用,板蓝根的加入对BSA构象产生了影响。对板蓝根与HSA的相互作用也进行了研究。 3.研究了知母水溶液的紫外和荧光光谱。实验结果表明,知母溶液中存在多种荧光组分。在不同浓度范围内体现不同荧光组分的荧光光谱。研究了知母与BSA体系的荧光光谱,实验表明二者之间存在能量转移,但由于组分干扰及自吸现象,药物荧光的敏化增强在光谱中不易辨认。更多还原

【Abstract】 With a long history, Chinese traditional medicine plays an important role in the fields of medicine not only in China but also in all over the world. With the development of science and technology, the way to modernization research of Chinese traditional medicine is becoming more and more crucial. Furthermore, among all kinds of methods, fluorescence spectrum is an extensively used technology in study on the interaction between small organic molecules especially drugs and serum albumin. Moreover the applications of three-dimensional fluorescence spectrum will show great prospects.In this thesis, we studied the fluorescence spectrum of three Chinese traditional herds including Radix Angelicae Pubescentis (RAP), Radix Isatidis (RI) and Rhizoma Anemarrhenae (RA). Based on the fluorescence properties of these drugs, the interaction between drugs and serum albumin is investigated respectively.The main contents in this paper include:1. Fluorescence properties of RAP were studied. The results show that there are two different fluorescent constitutes in RAP alkaline solutions. The interaction between RAP and bovine serum albumin (BSA), human serum albumin (HSA) was studied. Energy transfer between BSA and RAP was observed in fluorescence spectrum, and combination of BSA with RAP was demonstrated in UV absorption spectrum. The effects of several experimental conditions on the interaction were investigated.2. Fluorescence spectrum of RI was studied. The interaction between RI and BSA was studied. Energy transfer between BSA and RI was observed in fluorescence spectrum, and combination of BSA with RI was demonstrated in UV absorption spectrum. The interaction between RI and HSA was investigated, too.3. Fluorescence spectrum and absorption spectrum of RA were studied. More than one fluorescent constitute in RA solutions were observed. Based on the results the interaction between RA and BSA was investigated.更多还原