【作者】 梁志锋；

【导师】 林军；

【作者基本信息】 广西医科大学 ， 药理学， 2005， 硕士

【摘要】 目的:探讨复方地归胶囊对实验性糖尿病大鼠糖、脂代谢紊乱的调节作用并初步探讨其机制。方法:Wistar 纯系雄性大鼠禁食24h 后,腹腔注射50mg.kg-1STZ,三天后测定空腹血糖(FBG),以FBG≥14mmol.L-1为糖尿病动物模型(DM)标准,DM 模型大鼠按FBG 浓度排序,用查随机数字表法将其随机分成5 组:糖尿病模型组、胰岛素组(Ins 1-2U.d-1.rat-1 iH.)、复方地归胶囊(FFDGC)高、中、低剂量(分别灌胃给予FFDGC 药粉2.4g.kg-1.d-1、1.2g.kg-1.d-1、0.6g.kg-1.d-1),每天上午给药一次,连续3 个月。期间观察记录大鼠饮食、饮水、体重、大小便等一般情况,并在相应时间采血测定空腹血糖(FBG)、药后2h 血糖(2hBG)、甘油三酯(TG)、游离脂肪酸(FFA)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、糖化血红蛋白(HbA1c)、血清肌酐(Cr)、尿素氮(BUN)、一氧化氮(NO)、总抗氧化能力(T-AOC)等指标。结果:给药后2h,FFDGC 高、中、低剂量组对血糖有一定降低作用(降低率分别为16.2%、14.3%和13.3%)并能改善糖耐量;给药3 个月后能降低血红蛋白糖化率(P<0.05),对胰岛素水平有一定提高,胰岛、肾脏形态有一定改善;同时FFDGC 能不同程度地降低血清TG、LDL-C、FFA、Cr,UA、NO(P<0.05～0.01);升高ALb,TP,T-AOC(P<0.05～0.01),有一定量效关系;对升高HDL-C 和降低TC 有一定趋势(P>0.05)。结论:复方地归胶囊有降低血糖、调节血脂紊乱、增强机体抗氧化能力、保护胰岛β细胞和肾脏的作用,对糖尿病尤其并发症的治疗有潜在的应用价值。更多还原

【Abstract】 Objective: To study the effects of FFDGC on the disorders of glucoseand lipid in diabetic rats.Methods:Rats were injected with STZ50mg.kg-1(dissolved in 0.1mol.L-1 sodium citrate buffer,pH4.2)intraperitoneally after being fasted for 24h.Three days later,thefasting blood glucose(FBG) was measured.The rats were considered asdiabetes mellitus(DM) when FBG≥14.0mmol.L-1.Then DM rats were dividedinto 5 groups at random:model group,insulin group(1-2U.rat-1.d-1 iH.),FFDGC high dose group(2.4g.kg-1.d-1 ig.),FFDGC middle dose group(1.2g.kg-1.d-1 ig.)and FFDGC low dose group (0.6g.kg-1.d-1 ig.).Duringexperement,the FBG,HbA1c,TG,TC,HDL-C,LDL-C,FFA,Cr,UA,BUN,NO,T-AOCwere measured in different times.Results:FFGDC could decrease serumglucose after administration.The serum TG,LDL-C,FFA,Cr,BUN,UA and NOwere decreased,but the serum Alb,TP,T-AOC levels were increasedsignificantly(P<0.05-0.01) in the FFDGC-treated rats.The serumHDL-C,TC were not changed significantly compared with DM group.Theseeffects were related to the doses of the drug.Conclusion:The resultssuggest that FFDGC may decrease serum glucose,TG,LDL-C,BUN,Cr,UA andNO levels,increase serum ALb,TP,T-AOC levels and protect isletβcellsin STZ-induced diabetic rats,indicating that FFDGC may have value inDM complications.更多还原