【作者】 王玉芝；

【导师】 蒲斌；

【作者基本信息】 山东中医药大学 ， 中医外科学， 2005， 硕士

【摘要】 目的:以经验方食管癌1号为代表方研究破瘀散结法诱导食管癌EC9706细胞凋亡的作用。方法:用光学显微镜、电子显微镜、流式细胞仪等技术观察细胞凋亡。结果:实验显示各剂量复方食管癌1号作用于体外培养的食管癌EC9706细胞均可诱导其发生凋亡,凋亡细胞表现为细胞固缩,核染色质聚集或碎裂、胞质空泡化等;流式细胞仪检测凋亡率各剂量组与空白对照组比较均显著优于空白对照组(P<0.05)。浓度为1g/ml和浓度为0.5g/ml中药提取液组分别与氟尿嘧啶组比较有显著性差异(P<0.05),且随药物浓度增大,细胞凋亡率增高,在0.1～1g/ml的浓度范围内有明显的剂量依赖性,即药物浓度与凋亡率呈正相关。结论:破瘀散结法能诱导食管癌EC9706细胞发生凋亡,细胞凋亡率呈现剂量依赖性。更多还原

【Abstract】 Objective: As the representation of esophageal carcinoma no.l decoction to observe the effect of the apoptotic of esophageal carcinoma EC9706 cells induced by PoYuSanJieFa.Method: Examined by light microsoope> transmitting electron microsoope and flow cytornetry.Result: Apoptotic of esophageal carcinoma EC9706 cells can be induced by esophageal carcinoma no.l decoction . Under electron microscope.apoptotic modes were seen:the chromatin of the nucleus agglutinated topleces and the organelles in the cytoplasm preserved complete. The apoptotic peak was Identified by flow cytometry.And there is a significant difference between the treatment group and control group(P<0.05).And a significant difference were found between the high concentrations (1g/ml and 0.5 g/ml) of treatment group and control group(P<0.05).The results also demonstrated that inhibitory effects of recipe were associated with the high percentage of bypodiploid cells(0.1 ~lg / ml).Conclusion: Apoptotic of esophageal carcinoma EC9706 cells can be induced by PoYuSanJieFa. The results also demonstrated that inhibitory effects of recipe were associated with the high percentage of bypodiploid cells.更多还原