【作者】 张海燕；

【导师】 邱丽娟； 罗淑萍；

【作者基本信息】 新疆农业大学 ， 生物化学与分子生物学， 2005， 硕士

【摘要】 土壤盐渍化是影响农业生产和生态环境的重要问题之一。因此提高作物耐盐性,培育耐盐品种,耐盐机理的研究及耐盐基因的定位分离受到人们越来越多的关注。由于大豆品种资源丰富,不同品种间耐盐性差异较大。这使培育大豆耐盐高产等优良性状聚合的品种成为可能。本研究以三个“耐盐品种×盐敏感品种”的F2群体为实验材料,利用SSR 标记技术,结合BSA 法,共筛选到10 个与大豆耐盐基因连锁的SSR 标记,结合大豆遗传连锁图谱分析,将大豆耐盐基因定位于N 连锁群的satt339 与sat304 之间,旨在为分子标记辅助选择育种创造条件。本实验在三个不同的杂交组合中,均将大豆耐盐基因定位在同一位置,但标记与基因之间的距离和位置在不同的群体中有所不同,这可能与实验群体的大小、随机抽样误差及亲本间遗传背景差异有关。因此选择合适的群体对基因定位有重大的影响。另外,利用目前在大豆中已经克隆的耐盐相关基因及EST 序列31 个,设计52 对引物,分别对大豆耐盐品种文丰7 号和盐敏感品种Union 的基因组DNA,RNA 进行检测。结果发现所设计的引物在基因组DNA 中扩增完全相同,但在RNA 水平发现了9 对引物有差异,这9 对引物可作为大豆耐盐基因表达水平的标记,为大豆种质耐盐性鉴定提供参考,并且为进一步阐明大豆耐盐机理奠定基础。更多还原

【Abstract】 Salinization of soil is a severe problem that has negative effects on farm production and ecological environment. The yield of soybean is severely affected by soil salinity. Enhancing the ability of soybean to survive salt condition is very important to improve the yield of soybean and the utility of the salinity soil. Much attention is paid to raising salt-tolerant plants, studying salt-tolerance mechanism,locating and isolating salt-tolerance genes. The purpose of this study was to map the salt-tolerant gene and create condition for molecular assisted selection in soybean. Three crosses of salt tolerant cultivar with susceptible cultivar were used as materials in this experiment. A resistant and a susceptible DNA bulk were created by pooling the DNA of 15 resistant and 15 susceptible F2 plants from the Wenfeng7 x Union F2 population, Jingdou33 x Hark F2 population, Tiefeng8 x Zaoshu6 F2 population respectively, Contrasting DNA bulks were screened with 422 pairs of simple sequence repeat (SSR) markers. We identified 10 marker that was related to salt tolerant gene in three population. The salt tolerant gene was located between satt339 and satt255 on molecular linkage group N, the genetic distance and position between marker and the gene is different among the three cross, which indicates that the number of population, the difference of genetic background between the two parents and random sampling error may affect result gene mapping. So , it is important to select suitable population to gene mapping. We designed 52 pairs of primers from salt-related gene and EST from Genbank. Those primers were detected in DNA and RNA that abstracted in Wenfeng 7(salt-tolerant) and Union(salt-sensitive). We identified 6 pairs of primers that related to salt-tolerant gene and it can be used as reference to identify the salt-resistance of varieties. On the other hand, this study provided helpful trace about the molecular mechanism of salt-tolerance in soybean.更多还原