【作者】 罗在柒；

【导师】 乙引；

【作者基本信息】 贵州师范大学 ， 植物学， 2005， 硕士

【摘要】 石斛作为重要的中药材资源,目前市场需求量不断增大。为了解决石斛资源的市场需求,提高石斛种植效率,本研究通过从野生金钗石斛和环草石斛中分离出内生真菌,通过建立植物与内生真菌之间的共生体系,观测植株和微生物生长状况,对所分离得到的真菌菌株进行生物学筛选,进一步从植物微生态学、植物生理化学的角度检测和探讨植物生长代谢状况,为石斛栽培种植和石斛菌剂研究提供基础资料。 本论文包括三个部分,即石斛属植物内生真菌的筛选和植物-微生物共生体系的建立、内生真菌对石斛生长的生理生态效应以及内生真菌诱导子对石斛生长的生理生态效应,研究内容及结果如下: [1]实验从金钗石斛和环草石斛分离、纯化得到菌株共 53 株,其中有 22 株能促进金钗石斛生长的,18 株对金钗石斛造成伤害,有 25 株明显促进环草石斛生长,18 株对环草石斛造成伤害。此外,对金钗石斛和环草石斛生长都起到促进作用的共有 9 株。 [2]从对 2 种石斛有促进作用的 9 个菌株中选取具有代表性的 3株菌株进行真菌侵入实验,并观察其对石斛生理生态的影响。结果表明,从生理生态学角度深入研究得到,内生真菌对提高植株叶片光合速率作用效果相当明显;菌株对环草石斛在 SOD 活性方面增强效果强于金钗石斛,尤其是菌株 120 和 037 环草石斛促进效果更为明显;内生真菌均能促进 POD 酶活性提高,但菌株 117 共生体系金钗石斛植株 POD 活性持续时间长于环草石斛;金钗石斛共生体系中更多还原

【Abstract】 The effect of inoculation between dendrobiumus and its endophytic fungus arestudied in this paper. To study the physiological effect of endophytic fungi ondendrobium genus, we isolated, purified and cultured the endophytic fungus from thewild Dendrobium nobile lindl. and D.loddigesii Rolfe.,and setted up a symbioticsystem for Dendrobium genus and its endophytic fungus to try to set up a new culturalmethod for cultivating techniques and to supply some theoretical basis fordendrobiumus conservation and utilization. The results show that there are 22 fungus can promote Dendrobium nobilelindl.growth and 25 to D.loddigesii Rolfe.respectively, the fungi of 029、037、041、063、102、113、117、120and 140 can increase yield of Dendrobium nobile lindl.and D.loddigesii Rolfe.. We screening 3 funguses inoculated with Dendrobium nobile lindl. andD.loddigesiiRolfe.to determine the effect of endophtic fungi on enzyme activities.Theresults showed that the biomass of plant encreasing, such as the chlorphyllin on leaf,the rate of photochemistry and the contents of soluble protein can be increased byusing endophytic fungi in viteo culture, comparision with the controls, and which canenhanced the enzyme activity at the begin of invade,such as SOD, POD, PAL,.Additional, the elicitor of fungi and can promote the yield and the activity ofadaptable enzyme in proper contents of elicitor. We have presented our points in this aspect that the entophytic fungi can enhancethe physiological and biochemical responses on host plants in symbiotic system asfungi invade the host plant, which regulate metabolism action. It is important in vitroculture to select a proper rest for control of the growth of fungus to establish abeneficial symbiotic relationship between plants and fungi. The results can promotethe relative biotechnology’s development.更多还原