【作者】 李莉；

【导师】 杨更亮；

【作者基本信息】 河北大学 ， 分析化学， 2005， 硕士

【摘要】 分子烙印技术是一种制备具有特定选择性和亲合性的分子识别材料的技术。其良好的专一性和卓越的分子识别能力广泛用于高效液相色谱(HPLC)、毛细管电色谱(CEC)和薄层色谱(TLC)的固定相等。本文应用分子烙印技术分别以啶虫脒、头孢克洛和磺胺甲噁唑为模板分子,采用本体聚合法、原位聚合法制备了不规则型、连续棒型具有特定识别和分离性能的分子烙印聚合物,通过静态结合方法,考察了啶虫脒、头孢克洛烙印聚合物的选择结合能力;磺胺甲噁唑烙印聚合物用作高效液相色谱固定相,实现了磺胺甲噁唑与其结构类似物的分离,同时考察了分子识别过程中的影响因素。全文共分三章: 第一章:介绍了分子烙印技术的原理、进展状况及相关技术的应用。 第二章:利用分子烙印技术,分别以啶虫脒和头孢克洛为模板分子,采用本体聚合方法合成了对啶虫脒和头孢克洛具有高度选择性的模板聚合物。通过平衡吸附实验,评价了其对啶虫脒和头孢克洛的亲和力和选择性,与空白烙印聚合物相比,对啶虫脒和头孢克洛表现了很高的亲和力。通过与啶虫脒和头孢克洛类似物质在模板聚合物上的吸附行为比较,表明模板聚合物对啶虫脒和头孢克洛具有很好的选择性。 第三章:以磺胺甲噁唑为模板分子,采用原位聚合法制备了具有特定识别性能的棒状分子烙印聚合物。并通过高效液相色谱法研究了烙印聚合物的识别特性。作为色谱固定相,磺胺甲噁唑烙印聚合物和目标分子之间的相互作用除氢键作用外,也存在着疏水作用。同时考察含水量、流速对分离的影响,论证了分子烙印聚合物选择性专一的作用原理。结果表明,这种棒状烙印聚合物对模板分子及其类似物有很好的分离能力。更多还原

【Abstract】 Molecular imprinting technique (MIT) is a polymerizing technique to produce molecular recognition materials with high selectivity and affinity. It has draw increasing attention because of its higher molecular recognition ability and stability .MIT has been used in a wide rang of applications .such as chiral separation .solid phase extraction .sensors,etc .In this thesis ,A molecularly imprinted pilymer highly selective for acetaniprid and cefaclor were prepared by molecular imprinting technique. Its selectivity to acetaniprid and cefaclor were evaluated by batch adsorption experiments .Molecularly imprinted polymers based on sulfamethoxazole has been used as HPLC stationary phases,The preparation with close analogues was simple .There are three chapters in this thesisIn chapter one :The principle of MIP polymerization methods, application of this technique and its future developments were introduced.and the applications of HPLC on separation and HPLC-ELSD technology are also described..In chapter two : A molecularly imprinted polymer highly selective for acetaniprid and cefaclor were prepared by molecularly imprinted technique . Its selectivity to acetaniprid and cefaclor were evaluated by batch adsorption experiments, which showed that acetaniprid-imprinted polymer and cefaclor -imprinted polymer exhibited higher affinity to acetaniprid and cefaclor.In chapter three :Sulfamethoxazole was employed as a template for the preparation of in-situ molecularly imprinted polymer rods for use as stationary phases of liquid chromatography,High performance liquid chromatography was employed to study the recognition property of the resulting Mff ,It was shown that the Sulfamethoxazole imprinted polymer has specific selectivity and high affinity to the sulfamethoxazole.The interaction between the MIP and target molecules were found to arise from hydrogen bonding in the imprinted area and the hydrophobic interaction in the non-imprinted area .The effects of water and flow rate on the separation, The preparation procedure was simple,The results showed that was more strongly retained than its analogues in the imprinted polymer rod-type column.更多还原