【作者】 孙波；

【导师】 王振勇； 李建基；

【作者基本信息】 山东农业大学 ， 临床兽医学， 2005， 硕士

【摘要】 为了克服化学药物在治疗病毒性疾病上存在药物残留等缺点,本试验探索用水煎中草药制剂“消疫灵”治疗鸡新城疫这一新途径,并观察其治疗效果。 根据传统验方和以中兽医理论为依据,设计中药制剂“消疫灵”的配方,采用传统方法水煎制备出中药制剂“消疫灵”,进行体外抑菌、抗病毒试验。将NDV毒株接种在生长良好的单层细胞上,同时与不同稀释倍数的中药药液混合,在培养箱中继续培养,逐日进行观察,记录结果。结果表明:在单层细胞上,“消疫灵Ⅰ”稀释10~4倍数时,能够完全抑制NDV病毒的生长;“消疫灵Ⅱ”稀释到10~6倍时,可抑制病毒的生长。因此,该中药制剂在理论上可以抑制病毒的生长。将13种单味中草药和“消疫灵Ⅰ”、“消疫灵Ⅱ”分别制成1g/ml的中药煎剂,采用打孔法,将各味中药水煎制剂和2种方剂分别注入营养琼脂中,移入培养箱中培养24h,观察结果。发现13味中药及2种方剂的水煎提取物对大肠杆菌、金黄色葡萄球菌和链球菌均有较好的抑菌效果:“消疫灵Ⅰ”的抑菌直径分别为10mm、12mm、20mm;“消疫灵Ⅱ”的抑菌直径分别16mm、18mm、26mm。“消疫灵Ⅱ”的抑菌效果优于“消疫灵Ⅰ”。上述试验结果表明中药制剂“消疫灵”可以供临床试验使用。 在上述实验的基础上,选21日龄的非免疫健康雏鸡300只,随机分为6组,每组50只,按照以下方法进行试验:分为治疗1—1组、治疗1—2组、治疗2—1组、治疗2—2组、阳性对照组、阴性对照组,其中治疗组为口服中药组,阳性对照组只攻毒不喂中药,阴性对照组不攻毒不喂中药。每天观察试验鸡的精神状态、临床症状和死亡情况:分别于攻毒前1天、攻毒后1天、3天、7天、15天进行定期检测血液生化指标(血清总蛋白、血清白蛋白、血清球蛋白),并进行抗体滴度的监测和病理组织学观察。结果表明:“消疫灵”中药制剂可以提高机体的免疫能力,试验更多还原

【Abstract】 To solve the question of residual chemical drugs in chicken meat as treatment of Newcastle Disease, Chinese herbal complex Xiao-Yi-Ling was used in the experiment to explore a new way to treat ND with Chinese herb.The preparation of Xiao-Yi-Ling was designed based on proved recipes and Chinese Medicine Theories. The method of water extraction was used to make Xiao-Yi-Ling mixture.Then every kind concentration of Chinese herbal medicine solution was mixed with equivalent diluted NDV. Then they were cultured for 72 hours. The results showed that: Xiao-Yi-Ling Ⅰ preparation could prevent the NDV growth at dilution titer of 10000 and the Xiao-Yi-Ling Ⅱ preparation could prevent the NDV growth at dilution titer of 1000000. Then the single Chinese herbal preparation, the Xiao-Yi-Ling Ⅰ preparation and Xiao-Yi-Ling Ⅱ preparation was decocted to 1g/ml. By means of punching method, the single Chinese herbal medicine and the Xiao-Yi-Ling Ⅰ preparation and Xiao-Yi-Ling Ⅱ preparation was inoculated into culture flask of ugar with three kinds of bacteria ( Eschericha coli 、 Staphylococcus aureus and Streptococcus) and was cultured for 24 hours. The result indicated that the diameter of antibacterial activities of the preparation Ⅰ was from 10mm to 20mm, the preparation Ⅱ from 16mm to 26mm. It indicated that Chinese herbal preparation have advantage in preventing the bacteria growth. So Xiao-Yi-Ling preparation can be used in clinic.21-days healthy chickens (n=300) were divided into 6 groups (n=50, respectively) randomly. And the experiment was conducted as follows: the 6 groups were divided into 4 test groups which were infected with NDV and fed with Xiao-Yi-Ling preparation in the meantime, the control groups were notfed with preparation. The preparation was fed twice one day. Then the clinical signs and death index were mainly recorded everyday. Simultaneously the biochemical indexes of blood were measured in assigned time, including serum totalprotein, serum globulin and albumin. The index of immune organs and antibody titer against NDV were also measured. The results showed that: Xiao-Yi-Ling preparation could increase the immune function of chickens and the indexes of immune organs and the biochemical indexes of blood. On the 7th day, the antibody titer of chickens in the test groups reached the maximum(25 6), higher than the control groups’(p < 0.05); On the 15th day, the antibody titer of chickens in the test groups chickens reached its maximum(29 °), sharply higher than the control groups’(p < 0.05). The content of serum totalprotein of chickens in the test groups reached the aximum(39.5 ±3.36g/l); the content of globul chickens in the test groups reached its maximum(27.6 ± 1.01g/l) on the 15th day. So the test groups fed with Xiao-Yi-Ling preparation could promote immune function of body and antivirus activities.更多还原