【作者】 张丽红；

【导师】 蔡玉文；

【作者基本信息】 辽宁中医学院 ， 中西医结合基础， 2005， 硕士

【摘要】 目的:探讨并阐明中药复方益肠泰对人大肠癌细胞株CCL229的作用机制,为该药临床应用治疗大肠癌提供可靠的实验依据。方法:采用血清药理学方法,应用光镜、电镜技术和酶细胞化学方法观察中药益肠泰对大肠癌细胞株CCL229的增殖、凋亡和酶活性的影响。结果:①MTT结果显示,不同浓度药物血清对CCL229人大肠癌细胞株具有不同的抑制效应,且呈时间依赖性。大剂量中药组24小时的抑制率为54.78%,48小时的抑制率为65.6%。②光镜观察可见中药组细胞明显变圆,细胞核染色质凝集。③电镜观察,可见中药组大肠癌细胞出现早期、中期、晚期细胞凋亡的形态学改变。④电镜观察结果也显示中药组细胞表面的微绒毛明显减少,提示益肠泰具有降低大肠癌细胞侵袭能力的作用。⑤酶细胞化学法显示,中药组细胞,质膜标志酶Mg²⁺-ATP酶和内质网标志酶G-6-P酶的酶反应颗粒变小,数量减少。结论:①益肠泰具有抑制肿瘤细胞增殖的作用。②益肠泰具有诱导大肠癌细胞凋亡的作用。③益肠泰具有减低大肠癌细胞侵袭能力的作用④益肠泰具有降低大肠癌细胞Mg²⁺-ATP酶和G-6-P酶的活性的作用。更多还原

【Abstract】 Purpose: To study the effects of YI-CHANG-TAI on proliferation and activity of enzymes of colon cancer cell and explain the mechanism of its resisting-cancer function.Methods: In vitro, we cultured the cell strain of the human colon cancer cell CCL229. The inhibition of cell proliferation was determined by MTT method, the activity of G-6-Pase and Mg²⁺-ATPase was detected by enzyme cytochemistry, and the apoptosis was determined by light microscopy, electron microscopy.Results: （1） The serum contained Chinese herbs has evident inhibitory function to the human colon cancer cell CCL229, and the inhibitory effects have time dependence. The inhibiting rate is 54.78% after 24h culture, 65.6% after 48h culture. （2） The observation of LM shows the morphological change of the colon cells of Chinese herbs group. （3） The observation of EM shows the formation of the apoptotic body, which is the most characteristic change of the apoptosis cell. （4） The observation of EM also shows the decreased amount of the microvilli, which play a key role in the process of invasion and metastasis. （5） Enzyme cytochemistry method indicates that the response granules of G-6-Pase and Mg²⁺-ATPase in the colon cell become smaller after the treatment of serum contained Chinese herbs, the amount of the granules become fewer, the density is lower, some are negative. The activity of the enzyme decreased obviously.Conclusions: （1） YI-CHANG-TAI has the function of inhibiting theproliferation of the human colon cancer cell CCL229. （2）YI-CHANG-TAI can induce the human colon cancer cell apoptosis. （3）YI-CHANG-TAI can decrease the activity of the G-6-Pase andMg²⁺-ATPase of the human colon cancer cell. （4） YI-CHANG-TAI caninhibit human colon cancer cells’ ability of invasion and metastasis.更多还原