【作者】 范颖；

【导师】 刘辉；

【作者基本信息】 大连医科大学 ， 免疫学， 2005， 硕士

【摘要】 应激(stress)是指机体针对外界刺激的非特异性反应,即无论外界刺激的性质如何,机体总是做出大致相同的反应。心理应激可以改变一个人原有的健康生活方式和行为模式,因此,心理应激对于机体神经、内分泌、免疫系统的影响越来越受到人们的重视。许多研究表明,心理应激是影响身体健康的重要因素,与许多高发疾病,如:心脏病,高血压,糖尿病等,有着密切的联系。本文旨在探讨考试应激状态下人体免疫的变化及其与人格类型、MICA 基因多态性的关系。 从某医科大学在读二年级学生中随机选取 24 名,其中男性 13 名,女性 11 名,年龄在 19-21 岁之间;对其进行艾森克(EPQ)人格测试,可以将其分为外向稳定型、外向不稳定型、内向稳定型、内向不稳定型等类型。分别于考试前一天及开学第一周晨空腹采血,通过免疫散射比浊法测定学生免疫球蛋白及补体的含量,通过放射免疫技术(Radioimmunoassay,RIA)测定受试者血清中皮质醇(cortisol)的水平,同时采用聚合酶链反应(Polymerase Chain Reaction, PCR)技术检测“MHC-Ⅰ相关基因 A”(MICA)多态性,通过统计学方法计算应激免疫与遗传的关系及与人格的相关性。结果表明,期末考试血清皮质醇浓度为 549.7nmol/L,开学后第一周为 358.4nmol/L,考试前较考试后升高,有统计学意义(P<0.05)。考试前,补体 C3 浓度为更多还原

【Abstract】 Stress is a non-special reaction of body to exoteric stressor. No matterwhat is the quality of stressor, the body will do the same reactions. Whenthe organism faces some deleterious physiological stressor as chilliness,anoxic situation, and exsanguine situation, it will fight or flight. At thesame time, sympathetic nerve system (SNS) dose emergent mobilization,following fast heart beating, pulse increasing, bronchia dilatation, pupilexpansion, blood sugar rising, cruor function increasing and systemic bloodredistribution to renew inner condition balance. Selye called it generaladaptation syndrome (GAS) with its character that adrenocortical functionenhancing, thymus and lymph atrophy, mucous membrane of alimentarycanal bleeding or canker. GAS is usually divided into three courses:alarming, resisting and crocking up. In the first section, the body producesadapting reaction, not on the top level. If stressor still exists, it will comeinto resisting course. In this course the body will mobilize all resistingmechanism to resist damage stressor. If the resisting course did notcompensate dysfunction, then the third section will come and bringadjustment disease even death. Mason and Lazarus think that GAS is notdirect effect of physiological stimulation but psychological reaction. Theyemphasize that stress is not only lie on the character of external stressor butcognizing and response. Psychological stressor could change originalhealthy life stile and behavior pattern. So, the effect of psychological hurton nerves, incretion and immune system has been more recognized. Moreand more research show that psychological stress is the important factor tohuman heath. And there is specific relation between psychological stressand disease as heart disease, hypertension, diabetes and so on. Our research is aimed at the influence of test stress on students’humoral immunity functions with different personality, and its geneticbackground. We stochasticly select 24 college students in grade two, male13 female 11, aged between 19-21. EPQ test separate them extraversionstabilization, extraversion instability, endocentric stabilization, endocentricinstability. We exsanguinate from elbow vein in the day before test and thefirst weekend after holidays to detect immunoglobin and complement toknow humoral immunity functions. Paired-sample T test show that C3 andC4 obvious increase in the day before test (P<0.05). IgM and IgA levelsincrease in the day before test, but have no statistical significance than thatof the day after holidays (P<0.05). IgG decrease before test withnon-significance (P<0.05). The cortisol level was measured byradioimmuneassay (RIA) to know if they were in stress. The data showsthat there is obvious increase of cortisol in the day before test. (P<0.05) Wedetected MICA allelic polymorphism by PCR, and analysed thecorrelations with antibody and complement changes. The primer was basedon the ‘High Resolution Molecular Phototyping of MICA and MICBAlleles Using Sequence Specific Primers’. The primer sequences ofinternal positive control were derived from human tumor necrosis factoralpha(TNF α). The results show that there are 12 positive cases of PCR intotal as well as all the internal controls were successfully amplified, that isto say, 50% of alleles (12/24) were determined. The data shows that there isno obvious increase in cortisol, immunoglobins, and complements. Thus,we conclude that test stress influences substance basis ofincretion-immunity. Immune changes are not linked with polymorphism inMICA*008. The personality is not the factor to effect body’s reaction topsychological stress.更多还原