【作者】 谭晓华；

【导师】 杨磊； 李锋；

【作者基本信息】 石河子大学 ， 生物化学与分子生物学， 2005， 硕士

【摘要】 目的:卡波氏肉瘤（Kaposi’s Sarcoma,KS）是一种软组织多发性色素性血管肉瘤,具有明显的地域和种族特异性。经典型KS在我国主要发生于新疆地区的维吾尔和哈萨克族人。其病因目前尚不完全清楚。本研究旨在寻找新疆KS的流行病学相关因素,为进一步探讨其分子发病机制和新疆KS与AIDS相关型KS的防治创造条件。 方法:按同性别、同民族、年龄相差小于等于5岁、同一个居住地配对要求1:4选取对照,对高发于新疆维、哈族的KS进行采用流行病学病例对照研究,用x²检验分析其发病与环境及生活习惯的关系,并收集病例、对照的血液和肿瘤组织标本。对收集到的标本进行感染因子、血清细胞因子水平及免疫学等方面的检测。运用酶联免疫吸附法（Enzyme-Linked ImmunoadsorbentAssay,ELISA）法对收集的血清样本的血管内皮生长因子（Vascular Endothelial Growth Factor,VEGF）、肿瘤坏死因子（Tumor Necrosis Factor-α,TNF-α）、干扰素γ（Interferon-γ,IFN-γ）、白细胞介素（Interleukin,IL-6）及新喋呤（neopterin）和β₂-微球蛋白（β₂-miroglobin,β₂-MG）水平进行检测。采用套式聚合酶链反应（nested-PCR）检测收集到的血液和肿瘤组织的HHV8和EBV DNA以判断是否有这些病毒的感染。 结果:我们的流行病学调查未能证实KS发病与地理环境及生活习惯等方面的相关性:细胞因子VEGF、TNF-α、IFN-γ、IL-6、新喋呤和β₂-MG的血清水平增加,除IFN-γ（p=0.112）外,差异均有统计学意义（p值均小于0.05）;KS患者组HHV8病毒感染明显高于对照组（p<0.001）。 结论:①本流行病学调查资料没有发现KS患病与环境及生活方式的相关性;②KS 患者某些细胞因子水平异常,KS患者血清IL-6、TNF-α及VEGF水平均显著高于对照组;⑧KS 患者存在免疫激活状态,新蝶呤和β₂-MG的血清水平增加反应KS患者的体液和细胞免疫均处于激活状态;④人疱疹病毒8型（Human Herpesvirus 8,HHV8）病毒感染是KS发病的独立的危险因素。更多还原

【Abstract】 Objective: Kaposi’s sarcoma （KS） is a kind of soft tissue idiopathic multiple pigmented angiosarcoma. The occurrence of KS indicts the significance of speciality in race and territory. In China, classical KS mainly occurs in Uygur and Kazak ethic group in Xinjiang Autonomous Region. The etiological factor of KS is not clear yet. The aim of the study is to find epidemiology factors that are related to this malignant tumor, and offer more information for further description of the molecular mechanism of the pathogenesis of Xinjiang classical KS and AIDS associated KS.Methods: A 1:4 case control study was used and x² test was performed in this study to analysis the role of environment and life styles in the KS arise. The control groups were selected by the standard of the same sex, region, and age difference no more than 5 years compared to the corresponding KS cases. Meanwhile, we collected both the KS patients’ and controls’ blood and tissue samples. Then infectious factors, some cytokines and immune factors were tested. Vascular Endothelial Growth Factor （VEGF） , Tumor Necrosis Factor- α （TNF- α ）, Interferon （IFN- γ）, Interleukin （IL-6）, neopterin and β₂-Microglobin（ β₂-MG） serum level were tested by Enzyme-Linked Immunoadsorbent Assay （ELISA）. Nested-PCR was used to detect HHV8 and EBV DNA in all blood and tissue samples.Results: By Epidemiological Studies, we can not confirm the relationship between KS and environment or between KS and life style. In KS cases, some cytokines level are significant higher than those of the control groups, such as VEGF, TNF-α, IL-6, neopterin and β₂-miroglobin （β₂-MG） （p<0.05）, the IFN- γ level is also higher in KS cases but the significance has no statistic signification（p=0.112）; The HHV8 positive rate is significant higher in KS cases than those in controls （p<0.001） .Conclusions: ①The causality between KS and environment or between KS and life style were not confirmed; ② some cytokines are higher in KS cases than those of control groups; ③Some activation of immune system was found in KS cases; ④HHV8 infection is an independent risk factor for KS’ occurrence.更多还原