【作者】 徐军；

【导师】 李海峰；

【作者基本信息】 青岛大学 ， 神经病学， 2005， 硕士

【摘要】 目的 探讨在格林-巴利综合征(GBS)动物模型-实验性变态反应性神经炎(EAN)的发病过程中Th1和Th2类细胞反应的作用。 方法 ①实验动物随机分三组(FKG组、FK组、FG组),分别用神经节苷脂(GM1)、弗氏佐剂(FCA)、血蓝蛋白(FKH)三种免疫物以皮下注射的方式免疫实验动物(新西兰大白兔),免疫间隔时间为每20天免疫一次。共免疫3次。每10天记录动物肌力和收集血液标本1次。②采用ELISA法测定GM1抗体,采用反转录基因扩增一步法试剂盒(德国QIAGEN公司生产)进行反转录多聚酶链反应(RT-PCR)测定IFN-γ和IL-10的mRNA表达水平。③资料统计应用单因素方差分析、t检验的方法分别比较FKG、FK组、FG组各组内的差别、相同取血时间点各组之间,以及不同组间细胞因子表达量的差别。P<0.05,说明统计学上有差异;P<0.01说明统计学上有显著差异。 结果 动物出现一过性肌力下降,神经节苷脂滴度在免疫后逐渐增加,在40天左右达到高峰。细胞因子IFN-γ的表达量在免疫反应后逐渐增加,在免疫后的第40天左右达到高峰(疾病症状的最严重阶段),之后出现下降趋势,FKG组与FK组比较无差异,两组与FG组比较有差异;细胞因子IL-10表达量从免疫后逐渐开始增加.免疫早期FKG组与FK组无差异,但均与FG组有差异;免疫晚期KFG组与FK组有差异,FKG组的表达量较FK组略增高。 结论 Th1和Th2类细胞反应参与了实验性变态反应性神经炎(EAN)的发病过程。蛋白类载体可参与了Th1和Th2类细胞反应,而多糖类抗原也参与Th2类细胞反应。蛋白载体在GM1抗体产生中起重要作用。更多还原

【Abstract】 Objective: To investigate the roles of Th1 and Th2 cell reaction in the pathogenesis of EAN, an animal model of Guillain-Barre syndrome (GBS).Methods: (1) Eighteen New Zealand white rabbit) were divided into three group(FKG group, FK group and FG group). GM1, FCA and KLH were combined to FKG, FK and FG mixture respectively and used to immunize rabbits to make EAN. Immunization was made for three times. The interval time of immunization was in every 20 days. Clinical changes of EAN and blood samples were collected every 10 days. (3)Titers of GM1 antibodies were determined with ELISA methods and mRNA levels of IFN and IL-10 were determined with QIAGEN one step RT-PCR kit. The data were analyzed by using one way analysis of variance and T test. P<0.05 means that there was difference in statistics. P<0.01 means that there was significant difference in statistics.Result: Temporal decreased muscle strength was noted. GM1 IgG antibody began to rise after immunization and reach the climax after 40 days of immunization. The expression of IFN- γ mRNA increased gradually after first immunization, and reached the highest level at the 40 days after first immunization, and decreased after the climax. There was no difference between FKG and FK group. But there was significant difference between the former two groups and FG group. The expression of IL-10 mRNA began to increase gradually at 30~40 days after first immunization. There was no difference between FKG and FK group in early stage of immunization. There was difference between the former group and FG group. At the later stage of the immunization, there was difference between FKG and FK.Conclusion: Both Thl and Th2 reactions play important role in the pathogenesis of EAN induced by immunization of GM1. Protein carrier may induce both Thl and Th2 reactions. Polysarccraid antigens may also induce Th2 reaction. Protein carrier play an important role in the production of GM1 antibody.更多还原