【作者】 李琳；

【导师】 张志强；

【作者基本信息】 中国医科大学 ， 康复医学与理疗学， 2005， 硕士

【摘要】 目的 目前脑血管病已经是一种主要致死,致残的常见病。随着对缺血性脑卒中研究的深入,发现脑缺血后相当部分的血管能自然再通,或溶栓治疗后血流恢复再通,随之而来出现再灌注损伤。 有研究证明电刺激小脑顶核治疗可使大脑皮质局部脑血流增加,使脑梗塞体积缩小,肢体功能恢复明显改善。电刺激小脑顶核治疗后大脑皮质局部脑血流的增加,不伴随脑组织糖代谢率的改变。电刺激小脑顶核治疗可使TUNEL染色阳性细胞数明显减少,减少缺血神经元凋亡而起到神经保护作用。 超短波治疗非热效应使组织中的离子,偶离子,微粒之间的磨擦增加。有研究表明超短波的无热量治疗,可以减轻脑缺血缺氧,减轻细胞源性及血管源性脑水肿,对再灌注损伤起到保护作用。 本实验应用Wistar大鼠大脑中动脉栓塞再灌注(MCAO)模型,梗塞大脑中动脉2h后再灌注24h。采用Zea-Longa评分法进行评分。在再灌注18h分别用超短波治疗和经皮电刺激小脑治疗,观测脑细胞含水量、2,3,5-三苯基四氮唑溶液染色脑梗死体积和细胞中bcl-2、bax表达。 方法 成年健康Wistar大鼠65只。应用Zea-Longa线栓法,建立大脑中动脉闭塞再灌注(MCAO)动物模型,梗塞2小时再灌注。再灌注18h后分别给予电刺激小脑治疗和超短波治疗,对照组不给予任何治疗。再灌注24h后断头处死取脑。 取脑后分别测量脑水含量,脑梗死体积,bcl-2和bax的表达。采用更多还原

【Abstract】 OBJECTIVECerebrovascular disease is common illness causing death and disablity. With investigation of cerebral arterial thrombosis deeply, we find the blood vessel come to repeifusion injury after vessel is recanalization in cerebral ischemia and blood flow restore recanalization after thrombolysis.It is proved that fastigial nucleus stimulation can increase local cerebral blood flow of cerebral cortex, deflate cerebral infarction volume, and improve limbs function recovery. But the treatment do not change brain tissue sugar metabolic rate. Fastigial nucleus stimulation could decrease TUNEL dying cell obviously and has neuroprotective effect. Negative heat effect of ultrashort wave therapy is grit increase between ion, pair ion and corpuscle in tissue. The Negative heat effect is investigated to lessen cerebral ischemia, to lighten cellogenic and vasogenic brain edema, and to conservancy in reperfusion injury.Focal cerebral ischemia in Wistar rats is produced by 2 hours of occlusion of the middle cerebral artery (MCAO) followed by 24 hours of reperfusion. The neurological deficits evaluated by Zea - Longa Scale. The rats are then subject to transcetaneous electrical stimulation treatment or ultrashort wave treatment on their head at 18 hours after the end of reperfusion. The cerebral water content is derermined by wet/dry weighing method and the infarct volume measured by 2, 3,5- triphenyltetrazolium chloride staining technique. The activity of bcl - 2 and bax in ischemia tissue of brain and morphologic changes are tested.METHODSThere are 65 majority healthy Wistar rats used to MCAO with Zea - Longa method. The rats are then subject to transcetaneous electrical stimulation treatment or ultrashort wave treatment on their head at 18 hours after the end of reperfusion. The control group has not treatment. The rats are decapitated to recipe brain at 24 hours after the end of reperfusion.The brain is derermined by wet/dry weighing method and the infarct volume. The activity of bcl - 2 and bax in ischemia tissue of brain are tested. We use the T - test analysis the sample.RESULTS1. There is statistics difference between control group, transcetaneous electrical stimulation group and ultrashort wave group in wet/dry weighing method. Transcetaneous electrical stimulation and ultrashort wave can diminish wet/dry weighing method. There is not statistics difference between them.2. There is statistics difference between control group, transcetaneous electrical stimulation group and ultrashort wave group in the infarct volume. Transcetaneous electrical stimulation and ultrashort wave can diminish the infarct volume. There is not statistics difference between them.3. There is statistics difference between control group, transcetaneous electrical stimulation group and ultrashort wave group in the activity of bcl - 2 and bax in ischemia tissue of brain. Transcetaneous electrical stimulation and ultra-short wave can diminish apoptosis. There is not statistics difference between them.CONCLUSIONTranscetaneous electrical stimulation and ultrashort wave can decrease wet/ dry weighing method and the infarct volume, suppressor apoptosis in region is-更多还原