【作者】 刘勇；

【导师】 王恩普；

【作者基本信息】 中国医科大学 ， 眼科学， 2005， 硕士

【摘要】 目的 角膜新生血管(CNV)在角膜疾病中较为常见,可以由多种原因引起。新生血管化的角膜,改变了角膜正常结构,对其进行移植更易出现免疫排斥反应。Kringle1-5(K1-5)蛋白作为纤溶酶原的降解产物,具有特异性的抑制血管内皮细胞作用,从而抑制新生血管形成、生长。本研究局部应用人Kringle1-5蛋白滴眼液来观察其抑制角膜新生血管的效果。 方法 (1)建立动物模型:随机对28只健康新西兰白兔单侧角膜进行缝线法诱导角膜新生血管。(2)将实验动物随机化分2组,即K1-5实验组与PBS对照组:K1-5实验组给予配制好的1.5MG/L人K1-5蛋白滴眼液点眼,PBS对照组给予PBS液点眼,均为每日4次;同时对两组实验动物每日4次0.25%氯霉素眼液点眼预防感染。(3)每日在裂隙灯下观察实验眼角膜新生血管的出现时间、新生血管长度、面积以及角膜水肿情况。分别在第5、10天及第20天随机选择处死实验兔。取下角膜组织作病理(分别进行HE染色及免疫组织化学染色)。(4)对两组角膜新生血管形成时间、最长新生血管、新生血管面积进行统计学处理。 结果 (1)K1-5实验组在缝线法制备角膜新生血管模型后,最早于第3天(平均3.50±0.51天)可见新生血管芽进入角膜缘,新生血管在第9～15天生长最为旺盛;第20天时最长新生血管为4.38±0.27mm,新生血管面积27.51±4.19mm~2。而PBS对照组在缝线法制备角膜新生血管模型后,最早更多还原

【Abstract】 ObjectiveCorneal neovascularization ( CNV) was common in corneal diseases, which was caused by many reasons. Neovascularized cornea wound be rejected for the reason that the normal corneal structure was changed. As the degradation product of plasminogen, protein Kringle1-5 cound inhibit the vascular endothelial cell and had antiangiogenic ability. To observe the inhibitory effects on the corneal neovascularization , we used the human protein Kringlel - 5 eye drops in this study.Methods(1) Established the animal model; using sutures induced the neovascularization in the mono cornea of the healthy New Zealand rabbits. (2) Radomed 2 groups:the K1- 5 treatment group and the control group ; the treatment group recieved 1.5MG/L human Kl - 5 protein ,the control groupreceived the PBS eye drops. All eyes received eye drops 4 times a day and chloramphenicol eye drops 4 times a day, too. (3) Observed the length and area of corneal NV , and observed the edema of cornea also by using the slitlamp. Killed some rabbits in the 5th、10th day and the 20th day . Corneal tissue wound be in the pathology (HE and immunohistochemistry). (4) The time of NV entering the cornea, the longest length of NV and the area of NV wound be analysised by statistics.更多还原