【作者】 林广云；

【导师】 黎碧娜； 李树权；

【作者基本信息】 广东工业大学 ， 化学工程， 2005， 硕士

【摘要】 为了评价L99口服液排铅的效果,第一、研究适合的小鼠铅中毒模型。利用醋酸铅作为造模物质,使用KM小鼠,饮水[0.143g/L、0.286g/L、0.857g/L、2.571g/L(醋酸铅)]给予小鼠染毒,分别观察10d、20d、30d后测定血、肝、脑、肾、骨中铅的含量。观察染毒剂量、染毒时间对小鼠铅中毒模型的影响。结果显示,0.143g/L、0.286g/L、0.857g/L(醋酸铅)剂量饮水染毒30天,不造成实验小鼠的体重增长明显下降、不降低其食物利用率,可建立小鼠铅中毒模型。 第二、研究了L99口服液对铅中毒小鼠的排铅作用。把小鼠分为9组。组Ⅰ和组Ⅱ共用空白对照组,实验开始后,空白对照组小鼠饮用去离子水;模型对照组Ⅰ和各剂量组Ⅱ小鼠饮用0.143g/L醋酸铅水溶液;模型对照组Ⅱ和各剂量组Ⅱ小鼠饮用0.857g/L醋酸铅水溶液。同时低剂量组Ⅰ、Ⅱ组灌胃给予5.00mL(L99口服)/kgBW,中剂量组Ⅰ、Ⅱ组灌胃给予10.00mL(L99口服液)/kgBW和高剂量组Ⅰ、Ⅱ灌胃给予15.00mL(L99口服液)/kgBW,实验30天后,用石墨炉原子吸收分光光度法测定小鼠全血、肝、脑、肾、股骨中铅含量。结果表明,各剂量组Ⅰ小鼠肝、肾、股骨中铅含量比模型对照组Ⅰ低且有显著性差异(P<0.01),各剂量组Ⅰ小鼠全血中铅含量比模型对照组Ⅰ低且有显著性差异(P<0.05),高剂量组Ⅰ小鼠脑铅含量比模型对照组Ⅰ低且有显著性差异(P<0.01);各剂量组Ⅱ小鼠全血、肝、脑、肾、股骨中铅含量比模型对照组Ⅱ显著降低且有显著性差异(P<0.01)。说明L99口服液对铅中毒小鼠血液及脏器有明显的排铅作用。更多还原

【Abstract】 In order to evaluate the effect of the L99 oral liquid excreting lead, the first, the lead poisoning mouse model was researched. The lead acetate (PbAC) was used for a poisoning agent, KM mice were given drinking water containing 0. 143g/L 0. 286 g/L . 0. 857g/L. 2. 571g/L(PbAC), At 10th day 20th day 30th day, the concentration of lead in blood, liver, brain, kidney, bone were measured. The results showed that the average body weight and food utilization ratio of KM mice with 0.143g/L , 0. 286 g/L , 0. 857g/L (PbAC) exposure for 30 days werent markedly descent, this method can be used for establishing a lead poisoning mouse model.The second, the effect of the L99 oral liquid excreting lead was studied. The mice were divided into 9 groups. The blank control group was common for the group I and the group II , The blank control group drinking pure water from the experiment beginning, the model control I and the dose group I were given drinking water containing 0. 143g/L PbAC; the model control II and the dose group II were given drinking water containing 0. 857g/L PbAC; At the same time, 5. 00mL(L99 oral liquid)/kgBW was poured into the stomach of the low dose group I II; 10. 00mL(L99 oral liquid)/kgBW was poured into the stomach of the medium dose group I Hand 15. 00mL(L99 oral liquid)/kgBW was poured into the stomach of the high dose group I II for 30 days, then the concentration of lead in blood, liver, brain, kidney, bone were measured by GFAAS. The results showed that the concentration of lead in liver, kidney, bone of every dose group I were lower than the model control I (P<0. 01) , the concentration of lead in blood of every dose group I were lower than the model control I (P<0. 05) , the concentration of lead in brain of high dose group I was lower than the model control I (P<0. 01) ; the concentration of lead in blood, liver, brain, kidney, bone of every dose group II were lower than the model control I (P<0.01). These results showed that the L99 oral liquid has significant effects of excreting lead.更多还原