【作者】 乌日娜；

【导师】 张和平；

【作者基本信息】 内蒙古农业大学 ， 食品科学， 2005， 硕士

【摘要】 从内蒙古锡林郭勒盟牧区以传统方法制作酸马奶的牧民家庭采集的 16 份酸马奶样品中,分离到 49 株乳杆菌。经传统的形态学、生理生化特性研究及乳酸旋光性测定、菌体细胞壁中二氨基庚二酸分析等化学分类方法,分别鉴定为 Lb.casei(16株)、Lb.acidophilus Group(10 株)、Lb.plantarum(6 株)、Lactobacillus casei subsp. pseudoplantarum(3 株)、Lb.coryniformis subsp.coryniformis(5 株)、Lb.kefiranofaciens(2 株)、Lb.curvatu(s1 株)和异型乳酸发酵的 Lb.fermentum(1 株)、Weissella kandleri(1 株)以及未明确的 Lactobacillus sp.(2 株)等。 在此基础上,进一步利用分子生物学方法对菌株 Lb.casei.Zhang 和 ZL12-1 进行了 16S rDNA 基因的扩增与测序,并将结果与乳杆菌标准菌株的 16S rRNA/DNA 基因序列作同源性分析,建立系统发育树。结果表明,菌株 Lb.casei.Zhang 与标准菌株 Lb.casei ATCC 334 的同源性为 100%,菌株 ZL12-1 与标准菌株 Lb.gallinarum ATCC 33199 的同源性为 98%。结合系统发育树及 16S rDNA 的部分序列分析结果,可将菌株 Lb.casei.Zhang 判定为一株 Lb.casei subsp.casei,ZL12-1 判定为一株Lb.gallinarum,这与生理生化等传统分类鉴定方法所得结果一致。更多还原

【Abstract】 49 strains were isolated from 16 samples of traditionally home-made koumiss collected from the pastoral area in Inner Mongolia-XiLinGuoLe region. All of them belonged to Lactobacillus. According to the traditional morphological characters, physiological and biochemical properties, as well as chemical characteristics, they were identified to 10 species, namely, Lb.casei(16 strains), Lb.acidophilus Group(10 strains), Lb.plantarum(6 strains), Lb.kefiranofaciens(2 strains), Lb.curvatus (1 strains), Lb. paracasei subsp. pseudoplantarum(3 strains), Lb.coryniformis subsp.coryniformis(5 strains), Lb.fermentum(1 strains) and Weissella kandleri(1 strains), 2 strains had not been identified yet. To further differentiate Lb.casei.Zhang and ZL12-1, the genes of 16S rDNA were amplified in vitro and sequenced. Then a phylogenetic dendrogram was constructed by comparing these two sequences with other 16S rRNA/DNA sequences of Lactobacillus. The results indicated that the homology of Lb.casei.Zhang with Lb.casei ATCC 334 was 100%, and the similarity of ZL12-1 with Lb.gallinarum ATCC 33199 was 98%. Combined with analysis of the phyolgenetic tree and partial sequences of 16S rDNA , Lb.casei.Zhang was classified as Lb.casei subsp. casei, ZL12-1 belonged to Lb.gallinarum. The result was as same as that of traditional classification before.更多还原