【作者】 王标诗；

【导师】 刘慧霞；

【作者基本信息】 广西大学 ， 制糖工程， 2005， 硕士

【摘要】 酒精废液是一种高浓度有机废水,其成分十分复杂,而其中的很多组分都是可回收利用的资源。糖蜜酒精废液色素是一种天然色素,是制糖加工过程逐步累积而成的多种色素的混合物,它包括焦糖色素、美拉德色素和酚类色素等。据报道美拉德色素和酚类物色素都具有抗氧化活性。合成的抗氧化剂对人体健康有潜在的危害,因此,研究探索从酒精废液中提取天然抗氧化剂,探寻抗氧化物质的新来源,筛选出广谱、安全性高、抗氧化能力强的天然抗氧化剂具有重要的现实意义。本课题的主要研究内容及结果如下: 1.研究了甲醇对粉末状色素的浸提效果,通过单因素及正交试验得出甲醇对酒精废液中色素物浸提的最佳组合条件为:温度50℃,料液比1:20,时间90min。 2.通过对几种不同型号的树脂HPD-600、HPD-500、D301-R、NKA-Ⅱ、D296-R进行色素吸附试验,选出吸附效果较好的树脂型号HPD-600和D301-R,通过单因素及正交试验得出大孔树脂对酒精废液色素的最佳吸附条件:pH值7,温度45℃,吸附时间90min,在此条件下的吸附率高于80%。通过对乙醇洗脱剂浓度的选择得出40%的乙醇为较好的洗脱剂。 3.研究了pH值、温度、柠檬酸、苯甲酸钠、K⁺、Na⁺、Ca²⁺等金属离子、氧化剂、还原剂和散射光等对色素试样A和试样B的稳定性,结果表明对于试样A,在所研究的条件范围内,pH值、柠檬酸、氧化剂、还原剂及散射光对其影响较大。而这些条件对试样B的影响小于试样A。pH值、柠檬酸、氧化剂和还原剂对两种试样的影响要稍大于温度、苯甲酸钠和金属离子对两种试样的影响。 4.通过五种不同的体系:油脂、羟自由基、亚硝酸盐、超氧阴离子和更多还原

【Abstract】 Alcohol wastewater is a high-concentration organic wastewater with many components, but most of them can be reutilized. The pigment from molasses alcohol wastewater is a natural pigment that roots in molasses. It is a mixture with many components that is formed and accumulated in the sugar processing. It includes caramel pigment, maillard pigment and phenolic pigment. It is reported that maillard pigment and phenolic pigment both have the antioxidant activity. The synthetical antioxidants have the potential threaten to the health of people, so it is of great realistic significance to extract natural antioxidant from alcohol wastewater. We expect to find a more secure, more efficient, more inexpensive natural antioxidant. The main research contents and results of this thesis are reported as following:1. The extracting result of CH3OH on powdery pigment was studied at first. By the single factor and orthogonal experiment we got the best extracting condition of CH30H on powdery pigment :temperature 45℃, pH 7, time 90min .2. The macroporous resin HPD-600and D301-R with better effect was selected by the adsorptive experiment of five macroporous resin HPD-600、HPD-500、D301-R、NKA-II、D296-R on the pigment concentraction.By the single factor and orthogonal experiment we got the best adsorptive condition of macroporous resin : pH 7, temperature 45℃, time 90min . Its adsorptive rate is more than 80% in this condition.3. The stability of sample A and B was studied by pH , temperature, citric acid, sodium benzoate, metal ion, H2O2, NaNO2 and dispersion light. The result shows the effect of pH , H2O2, and NaNO2 is higher than that of temperature, citric acid, sodium benzoate, metal ion on the stability of pigment.4. The antioxidant activity of pigment of alcohol wastewater was studied by five different system (oil, 0H, N02-, 02- and DPPH) . The result shows that the two sample both have certain the antioxidant activity and their antioxidant activity enhances with the adding of concentraction, but their antioxidant effect is lower than TBHQ and Vc with the same concentration. The antioxidant activity of pigment sample A and B with 0. 04% concentration is lower than TBHQ 0. 02% concentration. Their IC50 is higher than Vc in the 0H, N02-, 02- and DPPH system.5. The pigments contain phenolic pigment and flavonoid pigment through qualitative, quantitative and infrared spectrum analysis. The content of flavonoid pigment and phenolic pigment in the sample B(11.561、146. 644mg/g) is higher in the sample A(5. 577、70. 224mg/g). Thin-layer chromatography experiment shows that the pigment contains five substances at least.6. The effect of antioxidant of pigments from alcohol wastewater may be the act of maillard pigment and phenolic pigment together.更多还原