【作者】 刘天龙；

【导师】 刘钟杰； 郭世宁；

【作者基本信息】 中国农业大学 ， 临床兽医学， 2005， 硕士

【摘要】 本试验通过体外抑菌试验和动物预试验,筛选出中药复方芩翘口服液。选用21日龄岭南黄鸡进行疗效试验,随机分为7个组,空白对照组、攻毒对照组、岑翘口服液高剂量组、中剂量组、低剂量组、中西复方组和西药组,每组33只;另选用27日龄岭南黄鸡进行机理研究,随机分为空白对照组、攻毒对照组、岑翘口服液治疗组,每组40只,于攻毒后6h、12h、24h、48h、72h、120h,各组随机抽取6只鸡,翼下静脉采血5ml,检测血清中NO、TNF-α含量。结果表明: 1.中药复方芩翘口服液及其组成药物黄连、黄苓、连翘、石榴皮均对O₇₈菌株大肠杆菌具有一定的体外抑菌作用,其最低抑菌浓度分别为208、167、167、104和125mg/ml。 2.攻毒对照组在攻毒后120-144h时仍有鸡死亡,西药组在96h时仍有鸡死亡,岑翘口服液低剂量组在72h时仍有死亡,而岑翘口服液高剂量组、中剂量组和中西结合治疗组在48h后便没有鸡只死亡。岑翘口服液各剂量组和中西复方组发生肝周炎、心包炎、腹膜炎的动物数量显著低于攻毒对照组（p<0.01）和西药组（p<0.05）。说明岑翘口服液对实验性鸡大肠杆菌病具有良好的治疗效果。 3.攻毒对照组血清中NO和TNF-α含量在攻毒后12h,显著高于空白对照组（p<0.01）。 4.岑翘口服液治疗组血清中NO含量从攻毒12h后,直到试验结束,皆显著低于攻毒对照组（p<0.01）;其血清中TNF-α含量在攻毒后12h、24h、48h和120h时,显著低于攻毒对照组（p<0.01）,而与空白对照组差异不显著。更多还原

【Abstract】 Through pretest, Qinqiao Oral Solution made of Chinese medicinal herbs with good bacteriostastic activity on E. coli O₇8was screened out. Then, 231 chickens with 21 days old were used as experimental animals, which were divided into seven groups at random, the control group, the challenge group, high-dose Qinqiao group, medium-dose Qinqiao group, low-dose Qianqiao group, Enrofloxacine group, and Enrofloxacine plus Qinqiao group, to observe the effects of Qinqiao Oral Solution on the treatment of artificial infected E. coli disease. In addition, 120 chickens with 27 days old were used being divided into 6 groups. At the 6h, 12h, 24, 48h, 72h and 96h after challenge with E. coli O₇8, 6 chickens from each group were selected at random and 5 ml blood was drawn from each chicken for the measurement of NO and TNF- α in the serum. The results are as follows:1. Qinqiao Oral Solution, as well as its components coptidis rhizoma, scutellaria, forsythia suspensa and granati cortex can restrain the growth of E. coli O₇8, and their MIC are respectively 208, 167, 167, 104 and 125 mg/ml, indicating that Qinqiao Oral Solution and its components have good bacteriostastic activity on E. coli O₇8.2. There were still chickens dead in the challenge group 120-244 hours later after challenge in the challenge group, 96 hours in the Enrofloxacine group, 72 hours in the low-dose Qinqiao group, 48 hours in the high-dose, medium-dose and Enrofloxacine plus Qinqiao groups. The numbers of chickens that have parahepatitis, pericarditis and peritonitis in the Qinqiao groups with different doses and Qinqiao plus Enrofloxacine group are significantly lower than that in the challenge group （p<0.01） and Enrofloxacine group （p<0.05）, suggesting that Qinqiao Oral Solution has good effects on the artificial infected E. coli disease in chicken.3. Twelve hours later after challenge, the levels of NO and TNF- α in the chickens of the challenge group are significantly higher than that of the control group, showing that NO and TNF- α are two important factors to induce the inflammations in E. coli disease in chicken.4. From 12 hours after challenge until the end of the experiment, the NO level of the Qinqiao group with three doses was significantly lower than that of the challenge group, as well as the TNF- α level was significantly lower than that of the challenge group, 12, 24, 48 and 120 hours later after challenge, indicating that decreasing the levels of NO and TNF- α to decrease the inflammation is one of the mechanism of Qinqiao Oral Solution in the treatment of E. coli disease in chicken.更多还原