【作者】 张强；

【导师】 郭素娟；

【作者基本信息】 北京林业大学 ， 森林培育， 2005， 硕士

【摘要】 甘草（Glycyrrhiza uralensis Fisch）是我国常用的大宗中药材,但是采收过程中会对生态环境带来负面影响。柠条（Caragana Microphylla Lam）为多年生落叶灌木,根系发达,抗逆性强,是防风固沙、保持水土的优良树种。本文试图探究用同气候区域内的甘草与柠条复合种植,既满足社会对甘草的需求,又通过柠条的保护作用减少甘草采收时对生态环境造成的破坏。首次采用组织培养的方法,力求避免土壤等外界因素影响,对甘草和柠条间的化感作用进行系统的研究,探讨两者复合种植的可行性,为实施甘草和柠条的复合种植奠定理论基础。结果如下: 1.柠条茎段在偏酸性（pH=6.5）条件下,MS 作为基本培养基,添加植物生长调节剂 6-BA0.2 mg.L^-1和IAA0.2 mg.L^-1增殖效果最佳;添加植物生长调节剂 IBA0.2mg.L^-1和NAA0.4 mg.L^-1生根效果最佳。 2.甘草茎段在偏酸性（pH=6.5）条件下,MS 作为基本培养基,添加植物生长调节剂 2,4D1.2mg.L^-1和6-BA1.0mg.L^-1增殖效果最佳,1/2MS+NAA0.5mg.L^-1为最优生根培养基。 3.甘草、柠条混合培养结果表明:甘草的株高、根长、鲜重等指标均优于其单独培养。这表明甘草与柠条存在互利关系,但通过培养基互换以及互滴叶片浸提液实验发现,小块（1cm³）培养基和低浓度(1mg.mL^-1)叶片浸提液均对甘草与柠条的生长有促进作用,而大块（2cm³）培养基和高浓度(5-50mg.mL^-1)叶片浸提液对甘草和柠条的生长均存在抑制作用。 4.通过高效液相色谱仪测定,柠条根系分泌物及叶片浸提液中主要含有酯类、萜类化合物,甘草根系分泌物及叶片浸提液中主要包括含有苯环的酚类、芴类、烯类化合物。但这些化合物的作用机理还有待于进一步研究。 5.虽然,甘草和柠条在生物学和生态学特性方面存在一定的差异,但两者混栽时表现为以促进作用为主的种间关系,只要采取适当的复合种植方式,就可以达到经济效益和生态效益兼得的目的。更多还原

【Abstract】 Glycyrrhiza uralensis Fisch is an in common use and big a Chinese herbal medicine material in our country, but adopts to accept the process inside will bring the negative influence to the ecosystem environment. Caragana Microphylla Lam livings the defoliation bush for many years, a department prospers, ising a good tree to defends the breeze sand and keeps Water and soil to grow. This text tries to investigate to use together the weather licorice in the district plants compound, then satisfy the society to the need of the licorice, pass again an of caragana the protection function reduces the licorice adopts to accept the breakage that result in to the ecosystem environment. Adopt the method of the tissue culture for the very first time, try hard for to avoid the outsider factor in etc. in soil affect, to licorice with caragana a research for turning feeling function proceeding system, study both possibility that compound plant, Result is as follows:1. Caragana caudex while being partial to the acidity（ pH=6.5） term, the MS conduct and actions is basic to culture medium, increase the plant growth regulates 6-BA0.2 mg.L^-1 and IAA0.2 mg.L^-1increases in value the result the best; Increasing the plant growth regulates an IBA0.2 Mg.L^-1 with NAA0.4 The mg.L^-1 takes root the result the best.2. Licorice caudex segment while being partial to the acidity（ pH=6.5） term, the MS conduct and actions is basic to culture medium, increase the plant growth regulates a 2,4 D1.2mg.L^-1 with 6- the BAl.0mg.L^-1 increases in value the result the best,1/2 MSs+ the NAA0.5mg/ L^-1 is a superior taking root to culture medium.3.The mixing planting both of Guralensis and C.microphylla result enunci — ation: The stub of the licorice is high, a long and fresh and heavy etc. index sign is all better than its educates alone. C.microphylla growth for exsitting with each other benefit relating to, but passing tissue culture with each other changing and with each other a leafs slice immersing lifting hour of liquid discovering, small piece（1 cm³） tissue culture with low den-sity(1 mg.mL^-1) leaf’s slice immersing lifting liquid all to licorice and C.microphylla the function, But the big piece（2 cm³） tissue culture represses the function with the high dens-ity(5-50 mg.mLs^-1) a growth for immersing lifting liquid to licorice and C.microphylla.4.Pass the liquid mutually the chromatogram apparatus analyse, root’s secretion andextraction from leafage of caragana contain phenol、Alkene、 Fluorene those framework buildup by phenyl. liquorice contain ester. The function mechanism of these compoundses still needs the further research.5.In conclusion, the biology and ecology characteristic of Guralensis is different from C.microphylla.The mixing planting model will not have obviously influence on medicinal. The aim of developing medicinal materials resources and protecting ecological environment will berealized.更多还原