【作者】 杨光；

【导师】 庄惠生；

【作者基本信息】 东华大学 ， 环境工程， 2005， 硕士

【摘要】 本文测定了酚类环境激素中双酚A、2,4-二氯苯酚和五氯酚对鲤鱼的急性毒性和亚急性毒性,研究了双酚A（BPA）、2,4-二氯苯酚（2,4-DCP）和五氯酚（PCP）对鲤鱼肝脏、肾脏、鳃中SOD酶、CAT酶的活性影响,并对其作用机理进行了初步的探讨。主要的研究结果有: 双酚A对鲤鱼的96小时LC₅₀为4.25mg/L（95%置信限为4.04～4.47mg/L）。BPA暴露对鲤鱼CAT酶和SOD酶活性均有显著影响。在相同浓度不同暴露时间（6h、12h、24h、72h、7d、12d）的情况下,CAT酶活性是先升高再降低的;SOD酶活性是先降低再升高的,并且随着时间的继续延长,SOD酶活性再次下降。在同一时间不同浓度(1/6、1/4、1/3、1/2LC₅₀)处理组中,CAT酶活性随浓度的升高先升高再降低;SOD酶活性随浓度的升高先降低再升高,并且随着时间的继续延长,CAT酶活性再次降低。 2,4-二氯苯酚对鲤鱼的96小时LC₅₀为13.9mg/L（95%置信限为11.8～16.6mg/L）。2,4-DCP暴露对鲤鱼CAT酶和SOD酶活性均有显著影响。在相同浓度不同暴露时间（6h、12h、24h、72h、7d、12d）的情况下,CAT酶活性是先降低再升高的,并且随着时间的继续延长,CAT酶活性再次下降;SOD酶活性是先升高再降低;在同一时间不同浓度(1/6、1/4、1/3、1/2LC₅₀)处理组中,CAT酶、SOD酶活性均随浓度的升高先升高再降低。 五氯酚对对鲤鱼的96小时LC₅₀为0.19mg/L（95%置信限为0.17～0.22mg/L）。PCP暴露对鲤鱼CAT酶和SOD酶活性均有显著影响。在相同浓度不同暴露时间（6h、12h、24h、72h、7d、12d）的情况下,CAT酶、SOD酶活性均是先升高再降低;在同一时间不同浓度(1/6、1/4、1/3、1/2LC₅₀)处理组中,CAT酶、SOD酶活性均随浓度的升高而降低。更多还原

【Abstract】 In this dissertation, acute and subacute toxicity of BPA, 2,4-DCP, PCP of phenolic environmental estrogens to Common Carp was determined, effects of BPA, 2,4-DCP, PCP on the activity of SOD and CAT in the liver, kidney, gill of Common Carp were studied, and the interaction mechanism was also investigated. The main results are as follows:The LC₅0 values of 96 hours BPA to Common Carp are 4.25 mg/L （4.04⁴.47mg/L）. The activities of SOD and CAT of the Common Carp were changed obviously after expose of BPA. For the groups treated with the same concentration but different time （6h, 12h 24h 72h 7d 12d ）, the activities of CAT increased initially and then decreased, and the activities of SOD decreased initially and then increased , and with the prolong of times, the activities of SOD decreased again. For the groups treated with the same time but different concentrations （1/6n 1/4 l/3 I/2 LC₅0） ,the activities of CAT increased initially and then decreased with concentrations, the activities of SOD decreased initially and then increased , and with the prolong of the times, the activities of SOD decreased again.The LC₅0 values of 96 hours 2,4-DCP to Common Carp are 13.9 mg/L （11.8¹6.6mg/L）. The activities of SOD and CAT of the Common Carp were changed obviously after expose of 2,4-DCP. For the groups treated with the same concentration but different time （6h, 12h 24h 72h 7d）, the activities of SOD increased initially and then decreased, and the activities of CAT decreased initially and then increased , and with the prolong of times, the activities of CAT decreased again. For the groups treated with the same time but different concentrations （1/6 l/4 1/3 l/2LC₅0） ,the activities of CAT and SOD increased initially and then decreased with concentrations. The LC₅0 values of 96 hours PCP to Common Carp are 0.19 mg/L （0.17⁰.22mg/L）. The activities of SOD and CAT of the Common Carp were changed obviously after expose of PCP. For the groups treated with the same concentration butdifferent time （6h 12h 24h 72h 7d）, the activities of CAT and SOD increased initially and then decreased. For the groups treated with the same time but different concentrations （l/6 l/4 1/3 , 1/2LC50） ,the activities of CAT and SOD increased with concentrations.更多还原