【作者】 金玉洁；

【导师】 周集体；

【作者基本信息】 大连理工大学 ， 环境工程， 2005， 硕士

【摘要】 本文综述了处理偶氮染料废水的多种方法,详细介绍了基因工程技术和生物强化方法的特点及其在废水处理中的应用。在此基础上,研究了四方面内容:基因工程菌JM109(pGEX-AZR)的培养发酵条件;基因工程菌JM109(pGEX-AZR)的脱色能力研究;受体菌E. coil JM109的脱色能力研究;以上二株菌的生物强化效果比较。基因工程菌JM109(pGEX-AZR)最佳培养发酵条件为:Na2HPO4(4. 26g/L),KH2PO4(2. 65g/L),MnSO4·7H2O(0. 002g/L), FeSO4·7H20(0. 01g/L),MgSO4·7H2O(0. 2g/L),CaCl2(0. 02g/L),NH4Cl(0. 5g/L),Amp.(50mg/L),葡萄糖(5g/L)、氯化铵(3g/L),pH为7. 5,温度为35℃;接种量10mL/L。基因工程菌JM109(pGEX-AZR)(GEM)缺氧条件下脱色效果强于好氧条件下;缺氧条件下对酸性红B、酸性橙Ⅱ、酸性红6B、酸性黑10B、酸性红G、酸性红GR均有较好的脱色效果,其适用范围广;对不同浓度的染料(50-5000mg/L)均有脱色效果;在pH6-8之间,25-40℃,盐度1-5%,均有较好的脱色效果;在考察范围的盐度和pH范围内符合一级动力学方程;基因工程菌E.coli JM109(pGEX-AZR)脱色过程是-N=N-断裂生成小分子芳香胺的过程。受体菌E. coli JM109缺氧条件下对偶氮染料的脱色效果强于好氧条件下;能耐受5000mg/L的染料浓度,脱色率达到80%;对酸性红B、酸性橙Ⅱ、酸性红6B、酸性黑10B、酸性红G、酸性红GR均有较好的脱色效果,其适用范围较广;在pH6-8之间,25-40℃,盐度1-5%之间对偶氮染料均有较好的脱色效果;对偶氮染料的脱色过程是-N=N-断裂生成小分子芳香胺的过程。生物强化试验中,基因工程菌JM109(pGEX-AZR)和E.coli JM109相对于活性污泥系统均有强化效果;基因工程菌的强化能力优于JM109,进水葡萄糖最佳浓度为1000mg/L,对酸性红B、活性红K-2BP、酸性品红6B有较好的强化作用。更多还原

【Abstract】 Some technologies to treat azo dye wastewater are introduced in this paper, especially the characters and application of genetic engineering technology and bioaugmentation. Four aspects are included in the paper, the optimization condition of JM109 (pGEX-AZR) fermantation, the the study on decolinzation of JM109 (pGEX-AZR), the study on decolorization of JM109, the bioaugmentation of two bacteria.The optimal fermentation condition of JM109 (pGEX-AZR): Na2HPO4 (4.26g/L) , KH2PO4(2.65g/L), MnSO4-7H2O(0.002g/L), FeSO4-7H2O(0.01g/L), MgSO4-7H2O(0.2g/L), CaCl2(0.02g/L), NH4Cl(0.5g/L), Amp.(50mg/L), glucose (5g/L), NH4Cl (3g/L), pH 7.5, temperature35℃, inoculation quality10mL/LUnder anaerobic condition, JM109 (pGEX-AZR) can decolorize more efficiently than aerobic. It can decolorize numerous azo dyes, such as acid red red B, acid orange II, acid black 10B, acid red G, acid red GR. The decolorization concentration scale is 50~5000mg/L. The optimal decolorization condition is pH6~8,25-40 ℃, salinity 1-5%. The decolorization accords with the first class kinetics equation.Under anaerobic condition, E. coli JM109 can decolorize more efficiently than aerobic. It can decolorize numerous azo dyes, such as acid red B, acid orange II, acid black 10B, acid red G, acid red GR. The decolorization rate is 80% at the concentration of 5000mg/L. The optimal decolorization condition is pH6~8, 25-40 ℃, salinity 1-5%.Compared with the activated sludge, the two systems bioaugmented by JM109 (pGEX-AZR) and E.coli JM109 treat azo dye wastewater more efficiently, especially JM109 (pGEX-AZR). The optimal glucose concentration of influx is 1000mg/L. JM109 (pGEX-AZR) can bioaugment the treat of acid red B, active red K-2BP and acid fuchsin 6B.更多还原