【作者】 马强；

【导师】 王长海； 雷海民； 周玉新；

【作者基本信息】 大连理工大学 ， 生物化工， 2005， 硕士

【摘要】 本论文对红车轴草和川续断的化学成分及质量进行了系统的研究。 第一部分 红车轴草的化学成分及质量研究 1 对红车轴草有效部位的制备工艺进行研究,建立了红车轴草总异黄酮的定量分析方法。 2 应用硅胶柱层析、Sephadex LH-20凝胶柱层析等方法从红车轴草中分离得到了8个化合物,经理化和波谱解析确定了它们的结构,分别为十八烷醇、5-羟基-7,4’-二甲氧基异黄酮、鹰嘴豆芽素A、樱黄素、芒柄花素、染料木素、芒柄花苷和大豆苷元,其中十八烷醇和5-羟基-7,4’-二甲氧基异黄酮为首次从该植物中得到。 3 采用液相色谱-质谱联用技术对红车轴草中异黄酮类化合物进行分析,得到了样品的紫外色谱图、总离子流图和相应色谱峰的质谱图,通过谱图解析,共鉴别出17种异黄酮类化合物。 4 以水蒸气蒸馏法从红车轴草中提取挥发油,采用气相色谱-质谱联用技术进行了化学成分的分析,共鉴定了挥发油中的81种成分,并用归—化法测定了其相对含量。 5 建立了同时测定红车轴草药材中大豆苷元、染料木素、芒柄花素和鹰嘴豆芽素A含量的高效液相色谱方法。 6 建立了以二极管阵列检测器和蒸发光散射检测器同时测定红车轴草提取物中鹰嘴豆芽素A和芒柄花素含量的方法。 7 提出了红车轴草提取物及成品的质量标准草案,为其质量控制提供了科学依据。 第二部分 川续断的化学成分及质量研究 1 对川续断有效部位的制备工艺进行了研究,建立了川续断总皂苷的定量分析方法。 2 以水蒸气蒸馏法从川续断中提取挥发油,采用气相色谱-质谱联用技术进行了化学成分的分析,共鉴定了57种成分,并用归—化法测定了其相对含量。 3 建立了测定川续断药材中川续断皂甙Ⅵ含量的高效液相色谱方法,并对不同产地的川续断药材进行了测定。 4 提出了川续断药材的质量标准草案,为其质量控制提供了科学依据。更多还原

【Abstract】 In this paper, the chemical constituents and quality of Trifolium pratense L. and Dipsacus asperoides C. Y. Cheng et T. M. Ai were studied.Part one: Studies on the chemical constituents and quality of Trifolium pratense L.1 The preparation technology of the effective part of Trifolium pratense L. was discussed. The quantitative analysis method for the determination of the total isoflavone components was established.2 8 compounds were isolated by chromatography with silica gel column and Sephadex LH-20 gel column, and then identified by physicochemical properties and structurally elucidated by spectral analysis. The compounds obtained were 1-octadecanol, 5-hydroxy-7,4’-dimethoxyisoflavone, biochanin A, prunetin, formononetin, genistein, ononin and daidzein, respectively. 1-octadecanol and 5-hydroxy-7,4’-dimethoxyisoflavone were found in this plant for the first time.3 The isoflavones components in Trifolium pratense L. were analyzed by HPLC-MS. The UV absorption chromatogram, total ion current chromatogram of the sample and the mass spectra of the corresponding chromatographic peaks were obtained, and the resolution of these mass spectra was elucidated. 17 isoflavone components were identified in Trifolium pratense L.4 The essential oil from Trifolium pratense L. was extracted by steam distillation, and then analyzed by GC-MS. 81 compounds in the essential oil were identified, and their amount were determined by normalization method.5 The HPLC method for the simultaneous determination of daidzein, geninstein, formononetin and biochanin A in Trifolium pratense L. was established.6 The HPLC method with evaporative light scattering detector (ELSD) and diode array detector (DAD) was established for the determination of biochanin A and formononetin in Trifolium pratense L. extract.7 The quality standard of Trifolium pratense L. extract and product were studied in the paper, which may provide scientific basis for quality control.Part two: Studies on the chemical constituents and quality of Dipsacus asperoides C.Y. Cheng etT.M.Ai1 The preparation technology of the effective part of Dipsacus asperoides C. Y. Cheng et T. M. Ai was discussed. The quantitative analysis method for the determination of the total saponin components was established.2 The essential oil from Dipsacus asperoides C. Y. Cheng et T. M. Ai was extracted by steam distillation, and then analyzed by GC-MS. 57 compounds in the essential oil were identified, and their amount were determined by normalization method.3 The HPLC method for the determination of saponin VI in Dipsacus asperoides C.Y. Cheng et T. M. Ai was studied, and the samples of different habitats were analyzed.4 The quality standard of Dipsacus asperoides C. Y. Cheng et T. M. Ai was studied in the research, which may provide scientific basis for quality control.更多还原