悬浮式生物芯片检测系统中光学系统的研究

【作者】 李晨；

【导师】 陆祖康；

【作者基本信息】 浙江大学 ， 光学工程， 2005， 硕士

【摘要】 生物芯片技术是将大量的生物反应信息以及许多不连续的分析过程集成在一个芯片上，从而实现对生物大分子的准确、快速和大信息量的检测和分析，能在很短的时间内完成上万次的生物化学反应检测，具有高通量、高集成度、并行分析、微型化的优点。 从探索新的检测方式到发展出新型的生物芯片，人们在不断的改进发展生物芯片技术。发展出了微阵列式生物芯片以及悬浮式生物芯片。 悬浮式芯片的独特设计使得它拥有常规的检测方法所不具备的特点，本课题在现有的悬浮式生物芯片技术的基础上提出了一种全新的检测技术方案：采用独特的液流装置使微球体并行流过二维的检测区域；采用脉冲氙灯瞬时曝光照明，配合CCD连续进行凝结成像，以获取荧光信息图像。系统可以实现不间断的大通量并行检测，大大的提高检测速度。 本论文的内容：首先，对悬浮式生物芯片检测系统的总体结构和工作流程进行初步分析，总结各组分单元的技术研究要点；然后，对检测系统中的光学系统部分进行细致的研究分析，将其分为照明系统、荧光收集成像系统、滤色系统三个部分，针对光学性能提出要求，为光学系统的具体设计实施给出理论基础，并根据理论分析对检测系统进行设计；文章着重针对照明系统进行分析，为了更有效的进行照明系统的设计，在计算机中建立模型，再计算机模拟的辅助下，提出并改进设计方案，并付诸实施。文章最后给出整个系统的实施检测结果，在对这些结果进行分析的基础上，评价现有方案，提出改进意见。更多还原

【Abstract】 Biochip technology integrates large numbers of bio-reaction information and many discontinuous analysis processes on a chip, to realize accurate, quick detection and analysis with large information, and to complete ten thousands of biochemistry reaction in a very short time, having the advantage of high flux, high degree of integration, parallel analysis and micromation.From the discovery of new detection method to the development of new biochip, people keep improving and developing biochip technology, having achieved micro array and suspending array.The special design of suspending array has unique characteristic that routine detection method does not have. This project proposes a brand-new way of detection technology on the basis of present suspending array technology; adopts a special flow device to make microsphere flow parallel across planar detection area; adopts instantaneous exposal illumination of xenon flash lamp, cooperating with the CCD to freezing catch a series of images, to get images of fluorescence information. This system can realize continuous parallel detection of large amount of flux, which greatly improved the speed of detection.Content of this paper: first of all, elementarily analyze the general structure and work process of suspending array detection system, and summarize technical research points for each unit; then study and analysis the optical system in the detecting system, which is divided into three parts of illumination system, fluorescence collection and imaging system, and filtering system, among which proposes requirements for optical functions, gives theoretic basis to concrete design and implement of optical system, and designs the detecting system according to the academic analysis; this paper emphasizes on the analysis of illumination system, in order to process effectively the design of illumination system, models are established in the computer, under the aid of computer simulation, to propose and improve design project, then to implement. The end of this paper gives the implementing detection result of the whole system, onthe basis of result analysis, evaluating present project and proposing improvementsuggestions.更多还原