【作者】 陈钦华；

【导师】 薛立群；

【作者基本信息】 湖南农业大学 ， 养殖， 2004， 硕士

【摘要】 为了探讨和了解长沙地区奶牛乳房炎的主要致病性病原菌，为本病的防治提供科学依据，从而更有效地控制和防治奶牛乳房炎，提高长沙市乳源质量。本试验从2003年10月开始至2004年4月，历经7个月，分别无菌采取长沙地区三个主要奶牛场及专业户奶牛场的90份奶样进行奶牛乳房炎病原分离培养与鉴定。在这90份奶样中分离到三种细菌，根据培养特性、形态特征及生化试验鉴定为金色葡萄球菌、链球菌和大肠杆菌。试验并对分离得到的奶牛乳房炎病原葡萄球菌、链球菌、大肠杆菌进行了耐药性检测和治疗药物筛选。试验结果表明：头孢唑啉、头孢氨苄、阿米卡星、阿莫西林、氨苄西林、诺氟沙星等对葡萄球菌有显著的抑菌效果：阿莫西林、阿米卡星、头孢唑啉和林可胺类对链球菌有较为显著的抑菌效果；阿米卡星、新霉素、头孢唑啉对大肠杆菌高敏，呋喃妥因、头孢氨苄、卡那霉素、氧氟沙星和头孢拉啶对大肠杆菌中敏。更多还原

【Abstract】 This thesis attempts to explore the main cause of mastitis to provide scientific basis for the prevention of disease in order to control and prevent mastitis effectively, which will result in the improvement of the quality of milk origin in Changsha. During the seven-month experiment, the author collects ninety milk samples from three main cow farms and the cow farms of the farmers in order to execute the cultivation and evaluation of the split of disease origin of mastitis. The author got three genes from these milk samples. According to foster character, shape feature and bio-chemical experiment, they are named Staphylococcus, Streptococcus and Escherichia colitacillus. The experiment is also an attempt to make a anti-medicine test and a selection of medicine. The result shows that Staphylococcus have remarkable effect on the control of bacteria. Results also show that streptococcus is hyper-sensitive to Cefazolin(CZ)、 Cefalexin(CX)、 Amikacin(AN)、 Amoxicillin(AMX)、 Ampicillin(AM)、 Norfloxacin(NOR) ctc. And that Escherichia coli is medium-sensitive to Nitrofurantoin(FT)、 Cefalexin(CX)、 Kanamycin(KAN)、 Ofloxacin(OFL) and Ccfradine(CED).更多还原